Abstract
This study describes a simple, versatile approach for developing a nonviral gene carrier by adopting the highly efficient gene delivery properties of the adeno-associated virus (AAV). Specific viral peptides (r3.45-hepBD) extracted from AAV r3.45, which directly evolved to improve gene delivery capabilities in many cell types, were conjugated onto branched polyethylenimine (PEI) to form hybrid gene carriers. AAV r3.45 carries a sequence insertion (LATQVGQKTA; r3.45) within the heparin-binding domain (LQRGNRQA; hepBD), which ultimately comprises a novel sequence (LQRGNLATQVGQKTARQA; r3.45-hepBD) on the capsid. This sequence is hypothesized to be a crucial cue to enhance gene delivery efficiency. Consequently, the intimate interactions of the conjugated r3.45-hepBD with the glycosaminoglycans, including chondroitin sulfate, resulted in significantly enhanced cellular transfection of DNA/PEI-r3.45-hepBD complexes. The successful establishment of a nonviral system that is built with novel peptides will provide a powerful means for developing a substantial number of gene therapy applications.
| Original language | English |
|---|---|
| Pages (from-to) | 2136-2145 |
| Number of pages | 10 |
| Journal | Biomacromolecules |
| Volume | 14 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2013 Jul 8 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Materials Chemistry
- Polymers and Plastics
- Biomaterials
Cite this
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Integration of adeno-associated virus-derived peptides into nonviral vectors to synergistically enhance cellular transfection. / Kim, Jung Suk; Kim, Eunmi; Oh, Ji Seon; Jang, Jae-Hyung.
In: Biomacromolecules, Vol. 14, No. 7, 08.07.2013, p. 2136-2145.Research output: Contribution to journal › Article
TY - JOUR
T1 - Integration of adeno-associated virus-derived peptides into nonviral vectors to synergistically enhance cellular transfection
AU - Kim, Jung Suk
AU - Kim, Eunmi
AU - Oh, Ji Seon
AU - Jang, Jae-Hyung
PY - 2013/7/8
Y1 - 2013/7/8
N2 - This study describes a simple, versatile approach for developing a nonviral gene carrier by adopting the highly efficient gene delivery properties of the adeno-associated virus (AAV). Specific viral peptides (r3.45-hepBD) extracted from AAV r3.45, which directly evolved to improve gene delivery capabilities in many cell types, were conjugated onto branched polyethylenimine (PEI) to form hybrid gene carriers. AAV r3.45 carries a sequence insertion (LATQVGQKTA; r3.45) within the heparin-binding domain (LQRGNRQA; hepBD), which ultimately comprises a novel sequence (LQRGNLATQVGQKTARQA; r3.45-hepBD) on the capsid. This sequence is hypothesized to be a crucial cue to enhance gene delivery efficiency. Consequently, the intimate interactions of the conjugated r3.45-hepBD with the glycosaminoglycans, including chondroitin sulfate, resulted in significantly enhanced cellular transfection of DNA/PEI-r3.45-hepBD complexes. The successful establishment of a nonviral system that is built with novel peptides will provide a powerful means for developing a substantial number of gene therapy applications.
AB - This study describes a simple, versatile approach for developing a nonviral gene carrier by adopting the highly efficient gene delivery properties of the adeno-associated virus (AAV). Specific viral peptides (r3.45-hepBD) extracted from AAV r3.45, which directly evolved to improve gene delivery capabilities in many cell types, were conjugated onto branched polyethylenimine (PEI) to form hybrid gene carriers. AAV r3.45 carries a sequence insertion (LATQVGQKTA; r3.45) within the heparin-binding domain (LQRGNRQA; hepBD), which ultimately comprises a novel sequence (LQRGNLATQVGQKTARQA; r3.45-hepBD) on the capsid. This sequence is hypothesized to be a crucial cue to enhance gene delivery efficiency. Consequently, the intimate interactions of the conjugated r3.45-hepBD with the glycosaminoglycans, including chondroitin sulfate, resulted in significantly enhanced cellular transfection of DNA/PEI-r3.45-hepBD complexes. The successful establishment of a nonviral system that is built with novel peptides will provide a powerful means for developing a substantial number of gene therapy applications.
UR - http://www.scopus.com/inward/record.url?scp=84880077542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880077542&partnerID=8YFLogxK
U2 - 10.1021/bm4005854
DO - 10.1021/bm4005854
M3 - Article
C2 - 23701437
AN - SCOPUS:84880077542
VL - 14
SP - 2136
EP - 2145
JO - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 7
ER -