Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment

Andrew J. Percy, Jessica Tamura-Wells, Juan Pablo Albar, Kerman Aloria, Ardeshir Amirkhani, Gabriel D.T. Araujo, Jesus M. Arizmendi, Francisco J. Blanco, Francesc Canals, Jin Young Cho, Núria Colomé-Calls, Fernando J. Corrales, Gilberto Domont, Guadalupe Espadas, Patricia Fernandez-Puente, Concha Gil, Paul A. Haynes, Maria Luisa Hernáez, Jin Young Kim, Arthur KopylovMiguel Marcilla, Mathew J. McKay, Mehdi Mirzaei, Mark P. Molloy, Leanne B. Ohlund, Young Ki Paik, Alberto Paradela, Mark Raftery, Eduard Sabidó, Lekha Sleno, Daniel Wilffert, Justina C. Wolters, Jong Shin Yoo, Victor Zgoda, Carol E. Parker, Christoph H. Borchers

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC-MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC-MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.

Original languageEnglish
Pages (from-to)6-15
Number of pages10
JournalEuPA Open Proteomics
Volume8
DOIs
Publication statusPublished - 2015 Sep 1

All Science Journal Classification (ASJC) codes

  • Biochemistry

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    Percy, A. J., Tamura-Wells, J., Albar, J. P., Aloria, K., Amirkhani, A., Araujo, G. D. T., Arizmendi, J. M., Blanco, F. J., Canals, F., Cho, J. Y., Colomé-Calls, N., Corrales, F. J., Domont, G., Espadas, G., Fernandez-Puente, P., Gil, C., Haynes, P. A., Hernáez, M. L., Kim, J. Y., ... Borchers, C. H. (2015). Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment. EuPA Open Proteomics, 8, 6-15. https://doi.org/10.1016/j.euprot.2015.06.001