Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia

J. Kim, S. Y. Goo, H. J. Chung, H. W. Yang, Taisoon Yong, K. H. Lee, S. J. Park

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.

Original languageEnglish
Pages (from-to)138-144
Number of pages7
JournalParasitology Research
Volume98
Issue number2
DOIs
Publication statusPublished - 2006 Jan 1

Fingerprint

Giardia lamblia
Giardia
Proteins
proteins
Complementary DNA
Clone Cells
cell cycle
Two-Hybrid System Techniques
clones
Nuclear Proteins
cytoskeletal proteins
Cell Cycle Checkpoints
two hybrid system techniques
Complex Mixtures
Gene Library
Cell Cycle
polyclonal antibodies
cDNA libraries
Antigens
ribosomal RNA

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Kim, J., Goo, S. Y., Chung, H. J., Yang, H. W., Yong, T., Lee, K. H., & Park, S. J. (2006). Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia. Parasitology Research, 98(2), 138-144. https://doi.org/10.1007/s00436-005-0040-8
Kim, J. ; Goo, S. Y. ; Chung, H. J. ; Yang, H. W. ; Yong, Taisoon ; Lee, K. H. ; Park, S. J. / Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia. In: Parasitology Research. 2006 ; Vol. 98, No. 2. pp. 138-144.
@article{2563a903f1784bcf827ef140038f09c4,
title = "Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia",
abstract = "A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.",
author = "J. Kim and Goo, {S. Y.} and Chung, {H. J.} and Yang, {H. W.} and Taisoon Yong and Lee, {K. H.} and Park, {S. J.}",
year = "2006",
month = "1",
day = "1",
doi = "10.1007/s00436-005-0040-8",
language = "English",
volume = "98",
pages = "138--144",
journal = "Parasitology Research",
issn = "0932-0113",
publisher = "Springer Verlag",
number = "2",

}

Kim, J, Goo, SY, Chung, HJ, Yang, HW, Yong, T, Lee, KH & Park, SJ 2006, 'Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia', Parasitology Research, vol. 98, no. 2, pp. 138-144. https://doi.org/10.1007/s00436-005-0040-8

Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia. / Kim, J.; Goo, S. Y.; Chung, H. J.; Yang, H. W.; Yong, Taisoon; Lee, K. H.; Park, S. J.

In: Parasitology Research, Vol. 98, No. 2, 01.01.2006, p. 138-144.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia

AU - Kim, J.

AU - Goo, S. Y.

AU - Chung, H. J.

AU - Yang, H. W.

AU - Yong, Taisoon

AU - Lee, K. H.

AU - Park, S. J.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.

AB - A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.

UR - http://www.scopus.com/inward/record.url?scp=29144440892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29144440892&partnerID=8YFLogxK

U2 - 10.1007/s00436-005-0040-8

DO - 10.1007/s00436-005-0040-8

M3 - Article

VL - 98

SP - 138

EP - 144

JO - Parasitology Research

JF - Parasitology Research

SN - 0932-0113

IS - 2

ER -