Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in Nicotiana tabacum

Sang Sook Lee, Hye Sun Cho, Gyeong Mee Yoon, Joon Woo Ahn, Hyong Ha Kim, Hyunsook Pai

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Using a yeast two-hybrid system, we identified NtRpn3, a regulatory subunit of 26S proteasome, as an interacting protein of NtCDPK1 calcium-dependent protein kinase in Nicotiana tabacum. Rpn3 in yeast is an essential protein involved in proteolysis of cell cycle regulatory proteins, and the carrot homolog of Rpn3 was previously isolated as a nuclear antigen that is mainly expressed in the meristem. NtCDPK1 physically interacts with NtRpn3 in vitro in a Ca2+-independent manner and phosphorylates NtRpn3 in a Ca2+-dependent manner with Mg2+ as a cofactor. NtCDPK1 and NtRpn3 are co-localized in the nucleus, nuclear periphery, and around plasma membrane in vivo. Both NtCDPK1 and AtRpn3, an NtRpn3 homolog of Arabidopsis, are mainly expressed in the rapidly proliferating tissues including shoot and root meristems, and developing floral buds. Virus-induced gene silencing of either NtRpn3 or NtCDPK1 resulted in the phenotypes of abnormal cell morphology and premature cell death in newly emerged leaves. Finally, NtCDPK1 interacts with NtRpn3 in vivo as shown by co-immunoprecipitation. Based on these results, we propose that NtCDPK1 and NtRpn3 are interacting in a common signal transduction pathway possibly for regulation of cell division, differentiation, and cell death in tobacco.

Original languageEnglish
Pages (from-to)825-840
Number of pages16
JournalPlant Journal
Volume33
Issue number5
DOIs
Publication statusPublished - 2003 Mar 1

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Meristem
proteasome endopeptidase complex
protein kinases
Nicotiana tabacum
Tobacco
Cell Death
calcium
Nuclear Antigens
Cell Cycle Proteins
Two-Hybrid System Techniques
Daucus carota
Premature Mortality
cell death
Gene Silencing
Immunoprecipitation
Arabidopsis
nuclear antigens
Cell Division
Proteolysis
yeasts

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Lee, Sang Sook ; Cho, Hye Sun ; Yoon, Gyeong Mee ; Ahn, Joon Woo ; Kim, Hyong Ha ; Pai, Hyunsook. / Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in Nicotiana tabacum. In: Plant Journal. 2003 ; Vol. 33, No. 5. pp. 825-840.
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Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in Nicotiana tabacum. / Lee, Sang Sook; Cho, Hye Sun; Yoon, Gyeong Mee; Ahn, Joon Woo; Kim, Hyong Ha; Pai, Hyunsook.

In: Plant Journal, Vol. 33, No. 5, 01.03.2003, p. 825-840.

Research output: Contribution to journalArticle

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