Interaction of PRK1 receptor-like kinase with a putative elF2B beta-subunit in tobacco

S. W. Park, S. H. Yu, M. I. Kim, S. C. Oh, T. H. Kao, Hyunsook Pai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

PRK1, a receptor-like kinase that is expressed in pollen, pollen tubes, and ovaries, has been shown to play important roles in pollen development and embryo sac development in Petunia inflata. We have used the kinase domain of PRK1 as a bait in the yeast two-hybrid system to identify PRK1-interacting proteins. The screening resulted in isolation of a cDNA encoding a protein highly homologous to the human and yeast beta-subunit of translation initiation factor 2B (eIF2B-beta), which was designated NeIF2Bbeta. eIF2B is a guanine nucleotide exchange protein that functions in the regulation of translation in eukaryotic cells. Deletion mutants of NeIF2Bbeta were analyzed for their interaction with PRK1, and the results suggested that the N-terminal half of NeIF2Bbeta, especially the region between residue 103 and 235, is important for the interaction. This protein association was confirmed by in vitro binding assay of the recombinant NeIF2Bbeta and PRK1 proteins. Despite high sequence homology between NeIF2Bbeta and its yeast counterpart, the NeIF2Bbeta cDNA could not rescue the phenotype of the yeast mutant strain lacking the GCD7 gene encoding eIF2B-beta, when transferred into the mutant strain.

Original languageEnglish
Pages (from-to)626-632
Number of pages7
JournalMolecules and Cells
Volume10
Issue number6
DOIs
Publication statusPublished - 2000 Jan 1

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Tobacco
Phosphotransferases
Yeasts
Pollen
Proteins
Complementary DNA
Petunia
Pollen Tube
Peptide Initiation Factors
Two-Hybrid System Techniques
Guanine Nucleotides
Eukaryotic Cells
Sequence Homology
Embryonic Development
Ovary
Phenotype
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Park, S. W. ; Yu, S. H. ; Kim, M. I. ; Oh, S. C. ; Kao, T. H. ; Pai, Hyunsook. / Interaction of PRK1 receptor-like kinase with a putative elF2B beta-subunit in tobacco. In: Molecules and Cells. 2000 ; Vol. 10, No. 6. pp. 626-632.
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abstract = "PRK1, a receptor-like kinase that is expressed in pollen, pollen tubes, and ovaries, has been shown to play important roles in pollen development and embryo sac development in Petunia inflata. We have used the kinase domain of PRK1 as a bait in the yeast two-hybrid system to identify PRK1-interacting proteins. The screening resulted in isolation of a cDNA encoding a protein highly homologous to the human and yeast beta-subunit of translation initiation factor 2B (eIF2B-beta), which was designated NeIF2Bbeta. eIF2B is a guanine nucleotide exchange protein that functions in the regulation of translation in eukaryotic cells. Deletion mutants of NeIF2Bbeta were analyzed for their interaction with PRK1, and the results suggested that the N-terminal half of NeIF2Bbeta, especially the region between residue 103 and 235, is important for the interaction. This protein association was confirmed by in vitro binding assay of the recombinant NeIF2Bbeta and PRK1 proteins. Despite high sequence homology between NeIF2Bbeta and its yeast counterpart, the NeIF2Bbeta cDNA could not rescue the phenotype of the yeast mutant strain lacking the GCD7 gene encoding eIF2B-beta, when transferred into the mutant strain.",
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Interaction of PRK1 receptor-like kinase with a putative elF2B beta-subunit in tobacco. / Park, S. W.; Yu, S. H.; Kim, M. I.; Oh, S. C.; Kao, T. H.; Pai, Hyunsook.

In: Molecules and Cells, Vol. 10, No. 6, 01.01.2000, p. 626-632.

Research output: Contribution to journalArticle

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AU - Park, S. W.

AU - Yu, S. H.

AU - Kim, M. I.

AU - Oh, S. C.

AU - Kao, T. H.

AU - Pai, Hyunsook

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