Interactions of Marsh Orchid (Dactylorhiza spp.) and Soil Microorganisms in Relation to Extracellular Enzyme Activities in a Peat Soil

Hojeong Kang, C. FREEMAN

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The nature of the interactions between microbes and roots of plants in a peaty soil were studied in a laboratory-based experiment by measuring activities of β-glucosidase, phosphatase, N-acetylglucosaminidase, and arylsulphatase. The experiment was based on control (autoclaved), bacteria-inoculated, and plant (transplanted with Dactylorhiza) treatments, and samples were collected over 4 sampling intervals. Higher enzyme activities were associated with the bacteria-inoculated treatment, suggesting that soil enzyme activities are mainly of microbial origin. For example, β-glucosidase activity varied between 25-30μmol g-1 min-1 in the bacteria-inoculated samples whilst the activity of the control ranged between 4-12μmol g-1 min-1. A similar pattern was found for all other enzymes. At the end of the incubation, the microcosms were destructively sampled and the enzyme activities determined in bulk soil, rhizospheric soil, and on the root surface. Detailed measurement in different fractions of the peat indicated that higher activities were found in rhizosphere. However, the higher activities of β-glucosidase, N-acetylglucosaminidase, and arylsulphatase appeared to be associated with bacterial proliferation on the root surface, whilst a larger proportion of phosphatase appeared to be released from root surface.

Original languageEnglish
Pages (from-to)681-687
Number of pages7
JournalPedosphere
Volume17
Issue number6
DOIs
Publication statusPublished - 2007 Jan 1

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Dactylorhiza
glucosidases
peat soils
peat soil
soil microorganism
soil microorganisms
enzyme activity
marshes
marsh
arylsulfatase
bacteria
phosphatase
bacterium
soil
soil enzymes
sampling
peat
rhizosphere
microcosm
microorganisms

All Science Journal Classification (ASJC) codes

  • Soil Science

Cite this

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title = "Interactions of Marsh Orchid (Dactylorhiza spp.) and Soil Microorganisms in Relation to Extracellular Enzyme Activities in a Peat Soil",
abstract = "The nature of the interactions between microbes and roots of plants in a peaty soil were studied in a laboratory-based experiment by measuring activities of β-glucosidase, phosphatase, N-acetylglucosaminidase, and arylsulphatase. The experiment was based on control (autoclaved), bacteria-inoculated, and plant (transplanted with Dactylorhiza) treatments, and samples were collected over 4 sampling intervals. Higher enzyme activities were associated with the bacteria-inoculated treatment, suggesting that soil enzyme activities are mainly of microbial origin. For example, β-glucosidase activity varied between 25-30μmol g-1 min-1 in the bacteria-inoculated samples whilst the activity of the control ranged between 4-12μmol g-1 min-1. A similar pattern was found for all other enzymes. At the end of the incubation, the microcosms were destructively sampled and the enzyme activities determined in bulk soil, rhizospheric soil, and on the root surface. Detailed measurement in different fractions of the peat indicated that higher activities were found in rhizosphere. However, the higher activities of β-glucosidase, N-acetylglucosaminidase, and arylsulphatase appeared to be associated with bacterial proliferation on the root surface, whilst a larger proportion of phosphatase appeared to be released from root surface.",
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Interactions of Marsh Orchid (Dactylorhiza spp.) and Soil Microorganisms in Relation to Extracellular Enzyme Activities in a Peat Soil. / Kang, Hojeong; FREEMAN, C.

In: Pedosphere, Vol. 17, No. 6, 01.01.2007, p. 681-687.

Research output: Contribution to journalArticle

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