Interference of quorum sensing and virulence of the rice pathogen Burkholderia glumae by an engineered endophytic bacterium

Hyun Soo Cho, Soo Young Park, Choong Min Ryu, Jihyun F. Kim, Jong Guk Kim, Seung Hwan Park

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Many bacterial species are known to thrive within plants. Among these bacteria, a group referred to as endophytes provide beneficial effects to the host plants by the promotion of plant growth and the suppression of plant pathogens. Among 44 putative endophytic isolates isolated from surface-sterilized rice roots, Burkholderia sp. KJ006 was selected for further study because of a lack of pathogenicity to rice, a broad spectrum of antifungal properties, and the presence of the nifH gene, which is an indicator for nitrogen fixation. In an attempt to control Burkholderia glumae, a casual pathogen of seedling rot and grain rot of rice, an N-acyl-homoserine lactonase (aiiA) gene from Bacillus thuringiensis was introduced into Burkholderia sp. KJ006 given that the major virulence factor of Burkholderia glumae is controlled in a population-dependent manner (quorum sensing). The engineered strain KJ006 (pKPE-aiiA) inhibited production of quorum-sensing signals by Burkholderia glumae in vitro and reduced the disease incidence of rice seedling rot caused by Burkholderia glumae in situ. Our results indicate the possibility that a bacterial endophyte transformed with the aiiA gene can be used as a novel biological control agent against pathogenic Burkholderia glumae that are known to occupy the same ecological niche.

Original languageEnglish
Pages (from-to)14-23
Number of pages10
JournalFEMS Microbiology Ecology
Volume60
Issue number1
DOIs
Publication statusPublished - 2007 Apr 1

Fingerprint

Burkholderia
Quorum Sensing
virulence
Virulence
rice
pathogen
Bacteria
bacterium
endophyte
gene
Endophytes
seedling
disease incidence
Seedlings
pathogenicity
nitrogen fixation
biological control
host plant
Biological Control Agents
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Cho, Hyun Soo ; Park, Soo Young ; Ryu, Choong Min ; Kim, Jihyun F. ; Kim, Jong Guk ; Park, Seung Hwan. / Interference of quorum sensing and virulence of the rice pathogen Burkholderia glumae by an engineered endophytic bacterium. In: FEMS Microbiology Ecology. 2007 ; Vol. 60, No. 1. pp. 14-23.
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Interference of quorum sensing and virulence of the rice pathogen Burkholderia glumae by an engineered endophytic bacterium. / Cho, Hyun Soo; Park, Soo Young; Ryu, Choong Min; Kim, Jihyun F.; Kim, Jong Guk; Park, Seung Hwan.

In: FEMS Microbiology Ecology, Vol. 60, No. 1, 01.04.2007, p. 14-23.

Research output: Contribution to journalArticle

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