Interleukin-1β, -6 and Interferon-γ Productions in Patients Undergoing Continuous Ambulatory Peritoneal Dialysis

Kyu Hun Choi, In Hee Lee, Sug Kyun Shin, Hyun Jin Noh, Shin Wook Kang, Ho Young Lee, Dae Suk Han

Research output: Contribution to journalArticle

Abstract

In order to investigate cytokine productions in patients undergoing continuous ambulatory peritoneal dialysis (CAPD), we studied the production of interleukin (IL)-1β, -6, and interferon (IFN)-γ by cultured peripheral blood mononuclear cells (PBMC) in peritonitis-free CAPD patients. The correlation of cytokine production with plasma parathyroid hormone (PTH) and albumin levels was also evaluated. While the release of IL-1β was not markedly different from controls, the release of IL-6 from 24-hour cultured PBMCs was significantly greater than that of controls (Mean±S.D., IL-6: 2186.8±1217.9 pg/ml, vs 1516.3±767.9, p<0.05). The addition of lipopolysaccahride (LPS, 10 μg/ml) significantly stimulated IL-1β and -6 production of PBMCs in CAPD patients and controls, compared to an unstimulated condition. The LPS-induced IL-1β production was also not markedly different from controls, whereas LPS-induced IL-6 production was significantly higher than controls (IL-6: 13220.7±7177.4 vs 7411.4±1236.9, p<0.05). However, the percentage increases of IL-6 production stimulated with LPS in CAPD patients were not significantly different from controls (p>0.05). No difference of baseline IFN-γ was detected between CAPD patients and controls, but phytohemagglutinin (PHA, 10 μg/ml)-stimulated IFN-γ release was significantly higher in CAPD patients than controls (2425.9±1565.0 pg/ml vs 1364.0±755.1, p<0.05). There was no significant correlation between PTH and, IL-1β, -6, or IFN-γ production. On the other hand, a significant correlation was observed between the serum albumin level and LPS-stimulated IL-6 production (r=0.54, p<0.05). In conclusion, CAPD seems to partly induce activation of PBMCs with an enhanced release of IL-6 and IFN-γ, and CAPD patients with higher serum albumin levels tend to show higher IL-6 production in immune response.

Original languageEnglish
Pages (from-to)240-246
Number of pages7
JournalYonsei medical journal
Volume39
Issue number3
DOIs
Publication statusPublished - 1998

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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