Intermittent parathyroid hormone treatment can promote linear growth in the ovariectomized growing rat

Sungkil Lim, Young Jun Won, Du Hong Park, Dong Hwan Shin, Jong In Yook, Hyun Chul Lee, Kap Bum Huh

Research output: Contribution to journalArticle

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Abstract

To compare the effect of intermittent parathyroid hormone (PTH) treatment with that of estrogen treatment on epiphyseal growth in ovariectomized rats, 46 Sprague-Dawley female rats aged 9 - 10 weeks (about 200 - 220 g) were either ovariectomized or sham operated. From 6 weeks after ovariectomy (ovx), rats were daily injected with subcutaneous human recombinant PTH (1 - 84)-dosed 30 μg/kg (the low dose PTH- treated group) or 300 μg/kg (the high dose PTH-treated group), 17 β-estradiol (the 17 β- estradiol-treated group, 30 μg/kg) or vehicle (the ovx-alone group), 5 times a week for 4 weeks. The decalcified sections of the distal femoral epiphyseal plate were analyzed on light microscopy after H and E strain, and the lengths of the zones of proliferation, maturing and hypertrophic chondrocytes were measured. The length of the growth plate, the zone of proliferation and the zone of hypertrophic chondrocyte in the ovx-alone group were significantly shorter than those of the sham-operated group. The treatment of 17 β- estradiol speeded up to the differentiation of cells from proliferating chondrocytes to maturing and hypertrophic chondrocytes even though the length of the growth plate was comparable to that of the sham-operated group. Both low and high dose PTH treatments increased the length of the growth plate, and those lengths were comparable to that of the sham-operated group. The fractions of proliferating, maturing and hypertrophic zone in the low dose PTH-treated group were also comparable to those of the sham-operated group. However, high dose PTH treatment slowed down the differentiation of cells from proliferating chondrocytes to maturing and hypertrophic chondrocytes to a greater extent, and therefore the fraction of proliferating chondrocytes of the high dose PTH-treated group was larger than that of the low dose PTH- treated group (73.8±1.8 Vs 63.3±1.3%, p<0.005). From these results, we showed that intermittent PTH treatment could promote linear growth in the ovariectomized growing rat. We propose that PTH may be an alternative drug candidate for promoting linear growth of long bones without the risk for early closure of the growth plate.

Original languageEnglish
Pages (from-to)166-172
Number of pages7
JournalYonsei medical journal
Volume40
Issue number2
DOIs
Publication statusPublished - 1999 Jan 1

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Parathyroid Hormone
Chondrocytes
Growth Plate
Growth
Estradiol
Cell Differentiation
Bone Development
Ovariectomy
Thigh
Sprague Dawley Rats
Microscopy
Estrogens
Light

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lim, Sungkil ; Won, Young Jun ; Park, Du Hong ; Shin, Dong Hwan ; Yook, Jong In ; Lee, Hyun Chul ; Huh, Kap Bum. / Intermittent parathyroid hormone treatment can promote linear growth in the ovariectomized growing rat. In: Yonsei medical journal. 1999 ; Vol. 40, No. 2. pp. 166-172.
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abstract = "To compare the effect of intermittent parathyroid hormone (PTH) treatment with that of estrogen treatment on epiphyseal growth in ovariectomized rats, 46 Sprague-Dawley female rats aged 9 - 10 weeks (about 200 - 220 g) were either ovariectomized or sham operated. From 6 weeks after ovariectomy (ovx), rats were daily injected with subcutaneous human recombinant PTH (1 - 84)-dosed 30 μg/kg (the low dose PTH- treated group) or 300 μg/kg (the high dose PTH-treated group), 17 β-estradiol (the 17 β- estradiol-treated group, 30 μg/kg) or vehicle (the ovx-alone group), 5 times a week for 4 weeks. The decalcified sections of the distal femoral epiphyseal plate were analyzed on light microscopy after H and E strain, and the lengths of the zones of proliferation, maturing and hypertrophic chondrocytes were measured. The length of the growth plate, the zone of proliferation and the zone of hypertrophic chondrocyte in the ovx-alone group were significantly shorter than those of the sham-operated group. The treatment of 17 β- estradiol speeded up to the differentiation of cells from proliferating chondrocytes to maturing and hypertrophic chondrocytes even though the length of the growth plate was comparable to that of the sham-operated group. Both low and high dose PTH treatments increased the length of the growth plate, and those lengths were comparable to that of the sham-operated group. The fractions of proliferating, maturing and hypertrophic zone in the low dose PTH-treated group were also comparable to those of the sham-operated group. However, high dose PTH treatment slowed down the differentiation of cells from proliferating chondrocytes to maturing and hypertrophic chondrocytes to a greater extent, and therefore the fraction of proliferating chondrocytes of the high dose PTH-treated group was larger than that of the low dose PTH- treated group (73.8±1.8 Vs 63.3±1.3{\%}, p<0.005). From these results, we showed that intermittent PTH treatment could promote linear growth in the ovariectomized growing rat. We propose that PTH may be an alternative drug candidate for promoting linear growth of long bones without the risk for early closure of the growth plate.",
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Intermittent parathyroid hormone treatment can promote linear growth in the ovariectomized growing rat. / Lim, Sungkil; Won, Young Jun; Park, Du Hong; Shin, Dong Hwan; Yook, Jong In; Lee, Hyun Chul; Huh, Kap Bum.

In: Yonsei medical journal, Vol. 40, No. 2, 01.01.1999, p. 166-172.

Research output: Contribution to journalArticle

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T1 - Intermittent parathyroid hormone treatment can promote linear growth in the ovariectomized growing rat

AU - Lim, Sungkil

AU - Won, Young Jun

AU - Park, Du Hong

AU - Shin, Dong Hwan

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AU - Lee, Hyun Chul

AU - Huh, Kap Bum

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N2 - To compare the effect of intermittent parathyroid hormone (PTH) treatment with that of estrogen treatment on epiphyseal growth in ovariectomized rats, 46 Sprague-Dawley female rats aged 9 - 10 weeks (about 200 - 220 g) were either ovariectomized or sham operated. From 6 weeks after ovariectomy (ovx), rats were daily injected with subcutaneous human recombinant PTH (1 - 84)-dosed 30 μg/kg (the low dose PTH- treated group) or 300 μg/kg (the high dose PTH-treated group), 17 β-estradiol (the 17 β- estradiol-treated group, 30 μg/kg) or vehicle (the ovx-alone group), 5 times a week for 4 weeks. The decalcified sections of the distal femoral epiphyseal plate were analyzed on light microscopy after H and E strain, and the lengths of the zones of proliferation, maturing and hypertrophic chondrocytes were measured. The length of the growth plate, the zone of proliferation and the zone of hypertrophic chondrocyte in the ovx-alone group were significantly shorter than those of the sham-operated group. The treatment of 17 β- estradiol speeded up to the differentiation of cells from proliferating chondrocytes to maturing and hypertrophic chondrocytes even though the length of the growth plate was comparable to that of the sham-operated group. Both low and high dose PTH treatments increased the length of the growth plate, and those lengths were comparable to that of the sham-operated group. The fractions of proliferating, maturing and hypertrophic zone in the low dose PTH-treated group were also comparable to those of the sham-operated group. However, high dose PTH treatment slowed down the differentiation of cells from proliferating chondrocytes to maturing and hypertrophic chondrocytes to a greater extent, and therefore the fraction of proliferating chondrocytes of the high dose PTH-treated group was larger than that of the low dose PTH- treated group (73.8±1.8 Vs 63.3±1.3%, p<0.005). From these results, we showed that intermittent PTH treatment could promote linear growth in the ovariectomized growing rat. We propose that PTH may be an alternative drug candidate for promoting linear growth of long bones without the risk for early closure of the growth plate.

AB - To compare the effect of intermittent parathyroid hormone (PTH) treatment with that of estrogen treatment on epiphyseal growth in ovariectomized rats, 46 Sprague-Dawley female rats aged 9 - 10 weeks (about 200 - 220 g) were either ovariectomized or sham operated. From 6 weeks after ovariectomy (ovx), rats were daily injected with subcutaneous human recombinant PTH (1 - 84)-dosed 30 μg/kg (the low dose PTH- treated group) or 300 μg/kg (the high dose PTH-treated group), 17 β-estradiol (the 17 β- estradiol-treated group, 30 μg/kg) or vehicle (the ovx-alone group), 5 times a week for 4 weeks. The decalcified sections of the distal femoral epiphyseal plate were analyzed on light microscopy after H and E strain, and the lengths of the zones of proliferation, maturing and hypertrophic chondrocytes were measured. The length of the growth plate, the zone of proliferation and the zone of hypertrophic chondrocyte in the ovx-alone group were significantly shorter than those of the sham-operated group. The treatment of 17 β- estradiol speeded up to the differentiation of cells from proliferating chondrocytes to maturing and hypertrophic chondrocytes even though the length of the growth plate was comparable to that of the sham-operated group. Both low and high dose PTH treatments increased the length of the growth plate, and those lengths were comparable to that of the sham-operated group. The fractions of proliferating, maturing and hypertrophic zone in the low dose PTH-treated group were also comparable to those of the sham-operated group. However, high dose PTH treatment slowed down the differentiation of cells from proliferating chondrocytes to maturing and hypertrophic chondrocytes to a greater extent, and therefore the fraction of proliferating chondrocytes of the high dose PTH-treated group was larger than that of the low dose PTH- treated group (73.8±1.8 Vs 63.3±1.3%, p<0.005). From these results, we showed that intermittent PTH treatment could promote linear growth in the ovariectomized growing rat. We propose that PTH may be an alternative drug candidate for promoting linear growth of long bones without the risk for early closure of the growth plate.

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