Interspecies microbial nexus facilitated methanation of polysaccharidic wastes

Shouvik Saha, Byong Hun Jeon, Mayur B. Kurade, Sanjay P. Govindwar, Pradip K. Chatterjee, Sang Eun Oh, Hyunseog Roh, Sean S. Lee

Research output: Contribution to journalArticle

Abstract

Compositional variations in organic wastes influence microbial abundancy and syntrophy during anaerobic digestion (AD), impacting the normal performance of digesters for methanation. Investigation of the microbial dynamics during AD following augmentation with polysaccharidic wastes (PW) revealed the association of effective digester performance and methane yields with the microbial nexus. Dominance of the acidogenic saccharolytic genera, Prevotella, Eubacterium, and Lachnoclostridium, enhanced the utilization of carbohydrates (54%) in PW-augmented digesters. Spearman's rs correlation showed dynamic interspecies interactions among acetogenic syntrophs, and that of iron oxidizers/reducers with acetoclastic and hydrogenotrophic methanogens. Propionate oxidizers in Chloroflexi (i.e., Bellilinea, Levilinea, and Longilinea) exhibited positive associations with acetoclastic methanogens. Increase in the population of acetoclastic methanogens (Methanosaeta, 77% and Methanosarcina, 9%) accelerated the methanogenic activity of PW-augmented digesters by 7 times during the exponential phase, increasing the methane yield (75%) compared to the control. Thus, microbial syntrophy facilitated the effective methanation of PW during AD process.

Original languageEnglish
Article number121638
JournalBioresource technology
Volume289
DOIs
Publication statusPublished - 2019 Oct 1

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Methanation
Methanogens
Anaerobic digestion
Methane
methane
Propionates
carbohydrate
Carbohydrates
iron
Iron
Nexus
digester
Association reactions
anaerobic digestion

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Saha, S., Jeon, B. H., Kurade, M. B., Govindwar, S. P., Chatterjee, P. K., Oh, S. E., ... Lee, S. S. (2019). Interspecies microbial nexus facilitated methanation of polysaccharidic wastes. Bioresource technology, 289, [121638]. https://doi.org/10.1016/j.biortech.2019.121638
Saha, Shouvik ; Jeon, Byong Hun ; Kurade, Mayur B. ; Govindwar, Sanjay P. ; Chatterjee, Pradip K. ; Oh, Sang Eun ; Roh, Hyunseog ; Lee, Sean S. / Interspecies microbial nexus facilitated methanation of polysaccharidic wastes. In: Bioresource technology. 2019 ; Vol. 289.
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Saha, S, Jeon, BH, Kurade, MB, Govindwar, SP, Chatterjee, PK, Oh, SE, Roh, H & Lee, SS 2019, 'Interspecies microbial nexus facilitated methanation of polysaccharidic wastes', Bioresource technology, vol. 289, 121638. https://doi.org/10.1016/j.biortech.2019.121638

Interspecies microbial nexus facilitated methanation of polysaccharidic wastes. / Saha, Shouvik; Jeon, Byong Hun; Kurade, Mayur B.; Govindwar, Sanjay P.; Chatterjee, Pradip K.; Oh, Sang Eun; Roh, Hyunseog; Lee, Sean S.

In: Bioresource technology, Vol. 289, 121638, 01.10.2019.

Research output: Contribution to journalArticle

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Saha S, Jeon BH, Kurade MB, Govindwar SP, Chatterjee PK, Oh SE et al. Interspecies microbial nexus facilitated methanation of polysaccharidic wastes. Bioresource technology. 2019 Oct 1;289. 121638. https://doi.org/10.1016/j.biortech.2019.121638