Abstract
Intestinal organoids are emerging tools in intestinal disease modeling and regeneration. Matrigel, a basement membrane matrix extracted from mouse sarcoma, is the gold standard material for intestinal organoid culture. Yet, the tumor origin of this matrix does not effectively provide organoids with native intestine-like microenvironments. To overcome this limitation, we demonstrate a decellularized small intestine-derived hydrogel reinforced with three extracellular matrix (ECM) proteins that are essential for intestinal development. To identify optimal conditions for intestinal organoid culture, we optimized the concentrations of the ECM hydrogel derived from decellularized intestine and three ECM proteins. The intestinal organoids grown in the established ECM hydrogels have shown similarity to those grown in Matrigel in terms of organoid forming efficiency, size, and gene expression patterns of intestine-specific markers. Our intestine-mimetic hydrogel could increase the feasibility of intestinal organoids for more clinically relevant applications in disease modeling and regenerative medicine.
| Original language | English |
|---|---|
| Pages (from-to) | 155-164 |
| Number of pages | 10 |
| Journal | Journal of Industrial and Engineering Chemistry |
| Volume | 107 |
| DOIs | |
| Publication status | Published - 2022 Mar 25 |
Bibliographical note
Funding Information:This work was supported by a grant (2021R1A2C3004262) from the National Research Foundation of Korea (NRF) funded by The Ministry of Science and ICT (MSIT), Republic of Korea. This work was supported by a grant (20009125) from the Korea Evaluation Institute of industrial Technology (KEIT) funded by the MSIT. This research was also supported by the Yonsei Signature Research Cluster Program of 2021-22-0014.
Publisher Copyright:
© 2021 The Korean Society of Industrial and Engineering Chemistry
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)