Intracellular calcium dynamics at the core of endocardial stationary spiral waves in Langendorff-perfused rabbit hearts

Liang Tang, Gyo Seung Hwang, Hideki Hayashi, Juan Song, Masahiro Ogawa, Kenzaburo Kobayashi, Boyoung Joung, Hrayr S. Karagueuzian, Peng Sheng Chen, Shien Fong Lin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In vitro models of sustained monomorphic ventricular tachycardia (MVT) are rare and do not usually show spiral reentry on the epicardium. We hypothesized that MVT is associated with the spiral wave in the endocardium and that this stable reentrant propagation is supported by a persistently elevated intracellular calcium (Cai) transient at the core of the spiral wave. We performed dual optical mapping of transmembrane potential (Vm) and Cai dynamics of the right ventricular (RV) endocardium in Langendorff-perfused rabbit hearts (n = 12). Among 64 induced arrhythmias, 55% were sustained MVT (>10 min). Eighty percent of MVT showed stationary spiral waves (>10 cycles, cycle length: 128 ± 14.6 ms) in the endocardial mapped region, anchoring to the anatomic discontinuities. No reentry activity was observed in the epicardium. During reentry, the amplitudes of Vm and Cai signals were higher in the periphery and gradually decreased toward the core. At the core, maximal Vm and Cai amplitudes were 42.95 ± 5.89% and 43.95 ± 9.46%, respectively, of the control (P < 0.001). However, the trough of the Vm and Ca i signals at the core were higher than those in the periphery, indicating persistent Vm and Cai elevations during reentry. BAPTA-AM, a calcium chelator, significantly reduced the maximal Ca i transient amplitude and prevented sustained MVT and spiral wave formation in the mapped region. These findings indicate that endocardial spiral waves often anchor to anatomic discontinuities causing stable MVT in normal rabbit ventricles. The spiral core is characterized by diminished Vm and Cai amplitudes and persistent Vm and Cai elevations during reentry.

Original languageEnglish
Pages (from-to)H297-H304
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume295
Issue number1
DOIs
Publication statusPublished - 2008 Jul 1

Fingerprint

Ventricular Tachycardia
Rabbits
Calcium
Endocardium
Pericardium
Voltage-Sensitive Dye Imaging
Isolated Heart Preparation
Cardiac Arrhythmias

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Tang, Liang ; Hwang, Gyo Seung ; Hayashi, Hideki ; Song, Juan ; Ogawa, Masahiro ; Kobayashi, Kenzaburo ; Joung, Boyoung ; Karagueuzian, Hrayr S. ; Chen, Peng Sheng ; Lin, Shien Fong. / Intracellular calcium dynamics at the core of endocardial stationary spiral waves in Langendorff-perfused rabbit hearts. In: American Journal of Physiology - Heart and Circulatory Physiology. 2008 ; Vol. 295, No. 1. pp. H297-H304.
@article{e06c4dafc38642ec93ec9f8e37bbc4e7,
title = "Intracellular calcium dynamics at the core of endocardial stationary spiral waves in Langendorff-perfused rabbit hearts",
abstract = "In vitro models of sustained monomorphic ventricular tachycardia (MVT) are rare and do not usually show spiral reentry on the epicardium. We hypothesized that MVT is associated with the spiral wave in the endocardium and that this stable reentrant propagation is supported by a persistently elevated intracellular calcium (Cai) transient at the core of the spiral wave. We performed dual optical mapping of transmembrane potential (Vm) and Cai dynamics of the right ventricular (RV) endocardium in Langendorff-perfused rabbit hearts (n = 12). Among 64 induced arrhythmias, 55{\%} were sustained MVT (>10 min). Eighty percent of MVT showed stationary spiral waves (>10 cycles, cycle length: 128 ± 14.6 ms) in the endocardial mapped region, anchoring to the anatomic discontinuities. No reentry activity was observed in the epicardium. During reentry, the amplitudes of Vm and Cai signals were higher in the periphery and gradually decreased toward the core. At the core, maximal Vm and Cai amplitudes were 42.95 ± 5.89{\%} and 43.95 ± 9.46{\%}, respectively, of the control (P < 0.001). However, the trough of the Vm and Ca i signals at the core were higher than those in the periphery, indicating persistent Vm and Cai elevations during reentry. BAPTA-AM, a calcium chelator, significantly reduced the maximal Ca i transient amplitude and prevented sustained MVT and spiral wave formation in the mapped region. These findings indicate that endocardial spiral waves often anchor to anatomic discontinuities causing stable MVT in normal rabbit ventricles. The spiral core is characterized by diminished Vm and Cai amplitudes and persistent Vm and Cai elevations during reentry.",
author = "Liang Tang and Hwang, {Gyo Seung} and Hideki Hayashi and Juan Song and Masahiro Ogawa and Kenzaburo Kobayashi and Boyoung Joung and Karagueuzian, {Hrayr S.} and Chen, {Peng Sheng} and Lin, {Shien Fong}",
year = "2008",
month = "7",
day = "1",
doi = "10.1152/ajpheart.00137.2008",
language = "English",
volume = "295",
pages = "H297--H304",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "1",

}

Intracellular calcium dynamics at the core of endocardial stationary spiral waves in Langendorff-perfused rabbit hearts. / Tang, Liang; Hwang, Gyo Seung; Hayashi, Hideki; Song, Juan; Ogawa, Masahiro; Kobayashi, Kenzaburo; Joung, Boyoung; Karagueuzian, Hrayr S.; Chen, Peng Sheng; Lin, Shien Fong.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 295, No. 1, 01.07.2008, p. H297-H304.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Intracellular calcium dynamics at the core of endocardial stationary spiral waves in Langendorff-perfused rabbit hearts

AU - Tang, Liang

AU - Hwang, Gyo Seung

AU - Hayashi, Hideki

AU - Song, Juan

AU - Ogawa, Masahiro

AU - Kobayashi, Kenzaburo

AU - Joung, Boyoung

AU - Karagueuzian, Hrayr S.

AU - Chen, Peng Sheng

AU - Lin, Shien Fong

PY - 2008/7/1

Y1 - 2008/7/1

N2 - In vitro models of sustained monomorphic ventricular tachycardia (MVT) are rare and do not usually show spiral reentry on the epicardium. We hypothesized that MVT is associated with the spiral wave in the endocardium and that this stable reentrant propagation is supported by a persistently elevated intracellular calcium (Cai) transient at the core of the spiral wave. We performed dual optical mapping of transmembrane potential (Vm) and Cai dynamics of the right ventricular (RV) endocardium in Langendorff-perfused rabbit hearts (n = 12). Among 64 induced arrhythmias, 55% were sustained MVT (>10 min). Eighty percent of MVT showed stationary spiral waves (>10 cycles, cycle length: 128 ± 14.6 ms) in the endocardial mapped region, anchoring to the anatomic discontinuities. No reentry activity was observed in the epicardium. During reentry, the amplitudes of Vm and Cai signals were higher in the periphery and gradually decreased toward the core. At the core, maximal Vm and Cai amplitudes were 42.95 ± 5.89% and 43.95 ± 9.46%, respectively, of the control (P < 0.001). However, the trough of the Vm and Ca i signals at the core were higher than those in the periphery, indicating persistent Vm and Cai elevations during reentry. BAPTA-AM, a calcium chelator, significantly reduced the maximal Ca i transient amplitude and prevented sustained MVT and spiral wave formation in the mapped region. These findings indicate that endocardial spiral waves often anchor to anatomic discontinuities causing stable MVT in normal rabbit ventricles. The spiral core is characterized by diminished Vm and Cai amplitudes and persistent Vm and Cai elevations during reentry.

AB - In vitro models of sustained monomorphic ventricular tachycardia (MVT) are rare and do not usually show spiral reentry on the epicardium. We hypothesized that MVT is associated with the spiral wave in the endocardium and that this stable reentrant propagation is supported by a persistently elevated intracellular calcium (Cai) transient at the core of the spiral wave. We performed dual optical mapping of transmembrane potential (Vm) and Cai dynamics of the right ventricular (RV) endocardium in Langendorff-perfused rabbit hearts (n = 12). Among 64 induced arrhythmias, 55% were sustained MVT (>10 min). Eighty percent of MVT showed stationary spiral waves (>10 cycles, cycle length: 128 ± 14.6 ms) in the endocardial mapped region, anchoring to the anatomic discontinuities. No reentry activity was observed in the epicardium. During reentry, the amplitudes of Vm and Cai signals were higher in the periphery and gradually decreased toward the core. At the core, maximal Vm and Cai amplitudes were 42.95 ± 5.89% and 43.95 ± 9.46%, respectively, of the control (P < 0.001). However, the trough of the Vm and Ca i signals at the core were higher than those in the periphery, indicating persistent Vm and Cai elevations during reentry. BAPTA-AM, a calcium chelator, significantly reduced the maximal Ca i transient amplitude and prevented sustained MVT and spiral wave formation in the mapped region. These findings indicate that endocardial spiral waves often anchor to anatomic discontinuities causing stable MVT in normal rabbit ventricles. The spiral core is characterized by diminished Vm and Cai amplitudes and persistent Vm and Cai elevations during reentry.

UR - http://www.scopus.com/inward/record.url?scp=49849102486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49849102486&partnerID=8YFLogxK

U2 - 10.1152/ajpheart.00137.2008

DO - 10.1152/ajpheart.00137.2008

M3 - Article

C2 - 18487432

AN - SCOPUS:49849102486

VL - 295

SP - H297-H304

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 1

ER -