Intracellular dielectric tagging for improved optical manipulation of mammalian cells

Patience Mthunzi, Woei Ming Lee, Andrew Clive Riches, Christian Tom A. Brown, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Optical micromanipulation of transparent microparticles such as cellular materials relies upon the application of optical forces that are crucially dependent on the refractive index contrast between the particle and the surrounding medium. We briefly review the application of optical forces for cell manipulation and sorting, highlighting some of the key experiments over the last twenty years. We then introduce a new technique for enhancing the dielectric contrast of mammalian cells, which is a result of cells naturally taking up microspheres from their environment. We explore how these intracellular dielectric tags can influence the scattering and gradient forces upon these cells from an externally applied optical field. We show that intracellular polymer microspheres can serve as highly directional optical scatterers and that scattering forces can enable sorting through axial guiding onto laminin-coated glass coverslips upon which the selected cells adhere. Such internal dielectric tagging presents a simple, inexpensive, sterile technique to enhance optical manipulation procedures for cellular material and may enable new sorting techniques within microfluidic systems.

Original languageEnglish
Article number5361341
Pages (from-to)608-618
Number of pages11
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume16
Issue number3
DOIs
Publication statusPublished - 2010 May 1

Fingerprint

Sorting
marking
manipulators
classifying
Cells
Microspheres
cells
foams
Scattering
scattering
Laminin
Microfluidics
Refractive index
Polymers
microparticles
Glass
refractivity
gradients
glass
polymers

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Atomic and Molecular Physics, and Optics
  • Materials Chemistry
  • Electrical and Electronic Engineering

Cite this

Mthunzi, Patience ; Lee, Woei Ming ; Riches, Andrew Clive ; Brown, Christian Tom A. ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Intracellular dielectric tagging for improved optical manipulation of mammalian cells. In: IEEE Journal on Selected Topics in Quantum Electronics. 2010 ; Vol. 16, No. 3. pp. 608-618.
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Intracellular dielectric tagging for improved optical manipulation of mammalian cells. / Mthunzi, Patience; Lee, Woei Ming; Riches, Andrew Clive; Brown, Christian Tom A.; Gunn-Moore, Frank J.; Dholakia, Kishan.

In: IEEE Journal on Selected Topics in Quantum Electronics, Vol. 16, No. 3, 5361341, 01.05.2010, p. 608-618.

Research output: Contribution to journalArticle

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