Intracellular translocation of superparamagnetic iron oxide nanoparticles encapsulated with peptide-conjugated poly(D,L lactide-co-glycolide)

Seung Jun Lee, Jong Ryul Jeong, Sung Chul Shin, Yong Min Huh, Ho Taek Song, Jin Suck Suh, Young Hwan Chang, Bong Sik Jeon, Jong Duk Kim

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In this study, we propose the use of iron oxide nanoparticle encapsulated with peptide-conjugated poly(D,L lactide-co-glycolide) (PLGA) as a potent intracellular carrier for diagnosis agent. The iron oxide (γ- Fe2 O3) nanoparticles were prepared by a chemical coprecipitation method of ferric and ferrous ions in an alkali solution. Arg peptide (RRRRRRRRCK-FITC) were conjugated to the PLGA via a simple coupling reaction between maleimide-derivatized PLGA and thiol-terminated Arg peptide. The γ- Fe2 O3 -PLGA-Arg-FITC nanoparticle was then prepared by an emulsification-diffusion technique. A confocal laser scanning microscopy revealed that the γ- Fe2 O3 -PLGA-Arg-FITC nanoparticle was effectively adsorbed onto the membrane of stem cells and delivered into the nuclei without cytotoxicity. Magnetic properties of the γ- Fe2 O3 -PLGA-Arg-FITC nanoparticle were observed by measuring the zero-field-cooledfield-cooled magnetization and magnetic hysteresis loop using a superconducting quantum interference device magnetometer from 5 K to 300 K.

Original languageEnglish
Article number10Q913
JournalJournal of Applied Physics
Issue number10
Publication statusPublished - 2005 May 15


All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

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