Abstract
In this study, we propose the use of iron oxide nanoparticle encapsulated with peptide-conjugated poly(D,L lactide-co-glycolide) (PLGA) as a potent intracellular carrier for diagnosis agent. The iron oxide (γ- Fe2 O3) nanoparticles were prepared by a chemical coprecipitation method of ferric and ferrous ions in an alkali solution. Arg peptide (RRRRRRRRCK-FITC) were conjugated to the PLGA via a simple coupling reaction between maleimide-derivatized PLGA and thiol-terminated Arg peptide. The γ- Fe2 O3 -PLGA-Arg-FITC nanoparticle was then prepared by an emulsification-diffusion technique. A confocal laser scanning microscopy revealed that the γ- Fe2 O3 -PLGA-Arg-FITC nanoparticle was effectively adsorbed onto the membrane of stem cells and delivered into the nuclei without cytotoxicity. Magnetic properties of the γ- Fe2 O3 -PLGA-Arg-FITC nanoparticle were observed by measuring the zero-field-cooledfield-cooled magnetization and magnetic hysteresis loop using a superconducting quantum interference device magnetometer from 5 K to 300 K.
Original language | English |
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Article number | 10Q913 |
Journal | Journal of Applied Physics |
Volume | 97 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2005 May 15 |
Bibliographical note
Funding Information:This research was partially supported by Center for Ultramicrochemical Process Systems project and by Ministry of Science & Technology project through the Creative Research Initiatives Project.
All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)