Abstract
After avulsion and replantation, teeth are at risk of bone and root resorption. The present study aimed to demonstrate that the intra‐nuclear transducible form of transcription modulation domain of p65 (nt‐p65‐TMD) can suppress osteoclast differentiation in vitro, and reduce bone re-sorption in a rat model of tooth replantation. Cell viability and nitric oxide release were evaluated in RAW264.7 cells using CCK‐8 assay and Griess reaction kit. Osteoclast differentiation was evaluated using quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) and tartrate‐re-sistant acid phosphatase (TRAP) staining. Thirty‐two maxillary rat molars were extracted and stored in saline (n = 10) or 10 μM nt‐p65‐TMD solution (n = 22) before replantation. After 4 weeks, specimens were scored according to the inflammatory pattern using micro‐computed tomography (CT) imaging and histological analyses. nt‐p65‐TMD treatment resulted in significant reduction of nitric oxide release and osteoclast differentiation as studied using PCR and TRAP staining. Further, micro‐CT analysis revealed a significant decrease in bone resorption in the nt‐p65‐TMD treatment group (p < 0.05). Histological analysis of nt‐p65‐TMD treatment group showed that not only bone and root resorption, but also inflammation of the periodontal ligament and epithelial insertion was significantly reduced. These findings suggest that nt‐p65‐TMD has the unique capabilities of regulating bone remodeling after tooth replantation.
Original language | English |
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Article number | 1987 |
Pages (from-to) | 1-15 |
Number of pages | 15 |
Journal | International journal of molecular sciences |
Volume | 22 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2021 Feb 2 |
Bibliographical note
Funding Information:Funding: This research was funded by Global Research laboratory (GRL) Program through the Na‐ tional Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) (NRF‐ 2016K1A1A2912755), the NRF grant funded by the Korea government (Ministry of Science, ICT& Future Planning) (NRF‐2017R1A2B2002537), Basic Science Research Program through NRF funded by the Ministry of Science, ICT & Future Planning (No. NRF‐2018R1A1A1A05018328), Basic Science Research Program through the NRF funded by the Ministry of Education(NRF‐ 2018R1D1A1B07041657), and the Yonsei University College of Dentistry Fund (6‐2019‐0006).
Funding Information:
This research was funded by Global Research laboratory (GRL) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) (NRF‐ 2016K1A1A2912755), the NRF grant funded by the Korea government (Ministry of Science, ICT& Future Planning) (NRF‐2017R1A2B2002537), Basic Science Research Program through NRF funded by the Ministry of Science, ICT & Future Planning (No. NRF‐2018R1A1A1A05018328), Basic Science Research Program through the NRF funded by the Ministry of Education(NRF‐ 2018R1D1A1B07041657), and the Yonsei University College of Dentistry Fund (6‐2019‐0006).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
All Science Journal Classification (ASJC) codes
- Catalysis
- Molecular Biology
- Spectroscopy
- Computer Science Applications
- Physical and Theoretical Chemistry
- Organic Chemistry
- Inorganic Chemistry