Intron retention-dependent gene regulation in Cryptococcus neoformans

Sara Gonzalez-Hilarion, Damien Paulet, Kyung Tae Lee, Chung Chau Hon, Pierre Lechat, Estelle Mogensen, Frédérique Moyrand, Caroline Proux, Rony Barboux, Giovanni Bussotti, Jungwook Hwang, Jean Yves Coppée, Yong Sun Bahn, Guilhem Janbon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The biological impact of alternative splicing is poorly understood in fungi, although recent studies have shown that these microorganisms are usually intron-rich. In this study, we re-annotated the genome of C. neoformans var. neoformans using RNA-Seq data. Comparison with C. neoformans var. grubii revealed that more than 99% of ORF-introns are in the same exact position in the two varieties whereas UTR-introns are much less evolutionary conserved. We also confirmed that alternative splicing is very common in C. neoformans, affecting nearly all expressed genes. We also observed specific regulation of alternative splicing by environmental cues in this yeast. However, alternative splicing does not appear to be an efficient method to diversify the C. neoformans proteome. Instead, our data suggest the existence of an intron retention-dependent mechanism of gene expression regulation that is not dependent on NMD. This regulatory process represents an additional layer of gene expression regulation in fungi and provides a mechanism to tune gene expression levels in response to any environmental modification.

Original languageEnglish
Article number32252
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Aug 31

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Cryptococcus neoformans
Alternative Splicing
Introns
Gene Expression Regulation
Genes
Fungi
Untranslated Regions
Proteome
Open Reading Frames
Cues
Yeasts
Genome
RNA
Gene Expression
Retention (Psychology)

All Science Journal Classification (ASJC) codes

  • General

Cite this

Gonzalez-Hilarion, S., Paulet, D., Lee, K. T., Hon, C. C., Lechat, P., Mogensen, E., ... Janbon, G. (2016). Intron retention-dependent gene regulation in Cryptococcus neoformans. Scientific reports, 6, [32252]. https://doi.org/10.1038/srep32252
Gonzalez-Hilarion, Sara ; Paulet, Damien ; Lee, Kyung Tae ; Hon, Chung Chau ; Lechat, Pierre ; Mogensen, Estelle ; Moyrand, Frédérique ; Proux, Caroline ; Barboux, Rony ; Bussotti, Giovanni ; Hwang, Jungwook ; Coppée, Jean Yves ; Bahn, Yong Sun ; Janbon, Guilhem. / Intron retention-dependent gene regulation in Cryptococcus neoformans. In: Scientific reports. 2016 ; Vol. 6.
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Gonzalez-Hilarion, S, Paulet, D, Lee, KT, Hon, CC, Lechat, P, Mogensen, E, Moyrand, F, Proux, C, Barboux, R, Bussotti, G, Hwang, J, Coppée, JY, Bahn, YS & Janbon, G 2016, 'Intron retention-dependent gene regulation in Cryptococcus neoformans', Scientific reports, vol. 6, 32252. https://doi.org/10.1038/srep32252

Intron retention-dependent gene regulation in Cryptococcus neoformans. / Gonzalez-Hilarion, Sara; Paulet, Damien; Lee, Kyung Tae; Hon, Chung Chau; Lechat, Pierre; Mogensen, Estelle; Moyrand, Frédérique; Proux, Caroline; Barboux, Rony; Bussotti, Giovanni; Hwang, Jungwook; Coppée, Jean Yves; Bahn, Yong Sun; Janbon, Guilhem.

In: Scientific reports, Vol. 6, 32252, 31.08.2016.

Research output: Contribution to journalArticle

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AU - Bussotti, Giovanni

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AU - Janbon, Guilhem

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Gonzalez-Hilarion S, Paulet D, Lee KT, Hon CC, Lechat P, Mogensen E et al. Intron retention-dependent gene regulation in Cryptococcus neoformans. Scientific reports. 2016 Aug 31;6. 32252. https://doi.org/10.1038/srep32252