Inverse correlation between mpsr1 e3 ubiquitin ligase and hsp90.1 balances cytoplasmic protein quality control

Jong Hum Kim, Tae Rin Oh, Seok Keun Cho, Seong Wook Yang, Woo Taek Kim

Research output: Contribution to journalArticle

Abstract

MISFOLDED PROTEIN SENSING RING1 (MPSR1) is a chaperone-independent E3 ubiquitin ligase that participates in protein quality control by eliminating misfolded proteins in Arabidopsis (Arabidopsis thaliana). Here, we report that in the early stages of proteotoxic stress, cellular levels of MPSR1 increased immediately, whereas levels of HEAT SHOCK PROTEIN90.1 (AtHSP90.1) were unaltered despite massively upregulated transcription. At this stage, the gene-silencing pathway mediated by microRNA 414 (miR414) suppressed AtHSP90.1 translation. By contrast, under prolonged stress, AtHSP90.1 was not suppressed, and instead competed with MPSR1 to act on misfolded proteins, promoting the destruction of MPSR1. Deficiency or excess of MPSR1 significantly abolished or intensified the suppression of AtHSP90.1, respectively. Similar to the MPSR1-overexpressing transgenic plants, the miR414-overexpressing plants showed an increased tolerance to proteotoxic stress as compared to the wild-type plants. Although the functional relationship between MPSR1 and miR414 remains unclear, both MPSR1 and miR414 demonstrated negative modulation of the expression of AtHSP90.1. The inverse correlation between MPSR1 and AtHSP90.1 via miR414 may adjust the setpoint of the HSP90-mediated protein quality control process in response to increasing stress intensity in Arabidopsis.

Original languageEnglish
Pages (from-to)1230-1240
Number of pages11
JournalPlant physiology
Volume180
Issue number2
DOIs
Publication statusPublished - 2019 Jun

Fingerprint

ubiquitin-protein ligase
Ubiquitin-Protein Ligases
Quality Control
quality control
MicroRNAs
microRNA
Proteins
proteins
Arabidopsis
Arabidopsis Proteins
Genetically Modified Plants
Gene Silencing
gene silencing
translation (genetics)
transgenic plants
Arabidopsis thaliana
transcription (genetics)

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

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title = "Inverse correlation between mpsr1 e3 ubiquitin ligase and hsp90.1 balances cytoplasmic protein quality control",
abstract = "MISFOLDED PROTEIN SENSING RING1 (MPSR1) is a chaperone-independent E3 ubiquitin ligase that participates in protein quality control by eliminating misfolded proteins in Arabidopsis (Arabidopsis thaliana). Here, we report that in the early stages of proteotoxic stress, cellular levels of MPSR1 increased immediately, whereas levels of HEAT SHOCK PROTEIN90.1 (AtHSP90.1) were unaltered despite massively upregulated transcription. At this stage, the gene-silencing pathway mediated by microRNA 414 (miR414) suppressed AtHSP90.1 translation. By contrast, under prolonged stress, AtHSP90.1 was not suppressed, and instead competed with MPSR1 to act on misfolded proteins, promoting the destruction of MPSR1. Deficiency or excess of MPSR1 significantly abolished or intensified the suppression of AtHSP90.1, respectively. Similar to the MPSR1-overexpressing transgenic plants, the miR414-overexpressing plants showed an increased tolerance to proteotoxic stress as compared to the wild-type plants. Although the functional relationship between MPSR1 and miR414 remains unclear, both MPSR1 and miR414 demonstrated negative modulation of the expression of AtHSP90.1. The inverse correlation between MPSR1 and AtHSP90.1 via miR414 may adjust the setpoint of the HSP90-mediated protein quality control process in response to increasing stress intensity in Arabidopsis.",
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Inverse correlation between mpsr1 e3 ubiquitin ligase and hsp90.1 balances cytoplasmic protein quality control. / Kim, Jong Hum; Oh, Tae Rin; Cho, Seok Keun; Yang, Seong Wook; Kim, Woo Taek.

In: Plant physiology, Vol. 180, No. 2, 06.2019, p. 1230-1240.

Research output: Contribution to journalArticle

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