Investigation of 3-D cell-based assays with structured illumination using a digital micromirror device

Jong Ryul Choi, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we demonstrate a fluorescence optical detection system with structured illumination using a digital micromirror device to investigate various cell-based assays. To study the performance of the optical detection system, we measured 2-D and 3-D assays of fluorescent microbeads and extended the results with mammalian cells. Through image reconstruction after structured illumination, we have improved fluorescence images by 32 times in terms of the size of axial point-spread function. The findings can be useful to supplement structured illumination microscopy that has been actively investigated for various applications.

Original languageEnglish
Title of host publicationFrontiers in Optics, FIO 2012
Publication statusPublished - 2012 Dec 1
EventFrontiers in Optics, FIO 2012 - Rochester, NY, United States
Duration: 2012 Oct 142012 Oct 18

Other

OtherFrontiers in Optics, FIO 2012
CountryUnited States
CityRochester, NY
Period12/10/1412/10/18

Fingerprint

illumination
cells
fluorescence
supplements
point spread functions
image reconstruction
microscopy

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

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Choi, JR & Kim, D 2012, Investigation of 3-D cell-based assays with structured illumination using a digital micromirror device. in Frontiers in Optics, FIO 2012. Frontiers in Optics, FIO 2012, Rochester, NY, United States, 12/10/14.

Investigation of 3-D cell-based assays with structured illumination using a digital micromirror device. / Choi, Jong Ryul; Kim, Donghyun.

Frontiers in Optics, FIO 2012. 2012.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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