Investigation of a nosocomial outbreak of Acinetobacter baumannii producing PER-1 extended-spectrum β-lactamase in an intensive care unit

Seokhoon Jeong, I. K. Bae, S. B. Kwon, Kyungwon Lee, DongEun Yong, G. J. Woo, J. H. Lee, H. I. Jung, S. J. Jang, K. H. Sung, Sang Hee Lee

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We investigated an outbreak of Acinetobacter baumannii in an adult intensive care unit of Kosin University Gospel Hospital in Busan, Republic of Korea. The outbreak involved 10 cases of infection by A. baumannii producing PER-1 extended-spectrum β-lactamase over a seven-month period, and was caused by a single pulsed-field gel electrophoresis clone. The epidemic isolates were characterized by slight synergy between clavulanic acid and cefepime. Isoelectric focusing of crude bacterial extracts detected two nitrocefin-positive bands with pI values of 8.0 and 5.3. Polymerase chain reaction amplification and characterization of the amplicons by restriction analysis and direct sequencing indicated that the epidemic isolates carried a blaPER-1 determinant. The epidemic isolates were characterized by a multidrug-resistant phenotype that remained unchanged over the outbreak, including penicillins, β-lactam/β-lactamase inhibitor, extended-spectrum cephalosporins and monobactams. Isolation of infected patients and appropriate carbapenem therapy were successful in ending the outbreak. Our report indicates that the blaPER-1 resistance determinant may become an emerging therapeutic problem.

Original languageEnglish
Pages (from-to)242-248
Number of pages7
JournalJournal of Hospital Infection
Volume59
Issue number3
DOIs
Publication statusPublished - 2005 Mar 1

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Acinetobacter baumannii
Disease Outbreaks
Intensive Care Units
Monobactams
Patient Isolation
Lactams
Clavulanic Acid
Republic of Korea
Carbapenems
Pulsed Field Gel Electrophoresis
Isoelectric Focusing
Cephalosporins
Complex Mixtures
Penicillins
Clone Cells
Phenotype
Polymerase Chain Reaction
Therapeutics
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Jeong, Seokhoon ; Bae, I. K. ; Kwon, S. B. ; Lee, Kyungwon ; Yong, DongEun ; Woo, G. J. ; Lee, J. H. ; Jung, H. I. ; Jang, S. J. ; Sung, K. H. ; Lee, Sang Hee. / Investigation of a nosocomial outbreak of Acinetobacter baumannii producing PER-1 extended-spectrum β-lactamase in an intensive care unit. In: Journal of Hospital Infection. 2005 ; Vol. 59, No. 3. pp. 242-248.
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Investigation of a nosocomial outbreak of Acinetobacter baumannii producing PER-1 extended-spectrum β-lactamase in an intensive care unit. / Jeong, Seokhoon; Bae, I. K.; Kwon, S. B.; Lee, Kyungwon; Yong, DongEun; Woo, G. J.; Lee, J. H.; Jung, H. I.; Jang, S. J.; Sung, K. H.; Lee, Sang Hee.

In: Journal of Hospital Infection, Vol. 59, No. 3, 01.03.2005, p. 242-248.

Research output: Contribution to journalArticle

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