Investigation of a nosocomial outbreak of imipenem-resistant Acinetobacter baumannii producing the OXA-23 β-lactamase in Korea

Byung Chan Jeon, Seok Hoon Jeong, Il Kwon Bae, Su Bong Kwon, Kyungwon Lee, Dongeun Young, Jung Hun Lee, Jae Seok Song, Sang Hee Lee

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

We investigated an outbreak of Acinetobacter baumannii in an intensive care unit and in the surgery, medicine, neurology, and urology wards of the Kosin University Gospel Hospital in Busan, Korea. The outbreak involved 36 cases of infection by A. baumannii producing the OXA-23 β-lactamase over an 8-month period and was caused by a single pulsed-field gel electrophoresis clone. The epidemic isolates were characterized by a modified cloverleaf synergy test. Isoelectric focusing of crude bacterial extracts detected one nitrocefin-positive band with a pI value of 6.65. PCR amplification and characterization of the amplicons by direct sequencing indicated that the epidemic isolates carried a blaOXA-23 determinant. The epidemic isolates were characterized by a multidrug resistance phenotype that remained unchanged over the outbreak, including penicillins, cephamycins, extended-spectrum cephalosporins, carbapenems, monobactams, and aminoglycosides. This study shows that the blaOXA-23 resistance determinant may become an emerging therapeutic problem.

Original languageEnglish
Pages (from-to)2241-2245
Number of pages5
JournalJournal of Clinical Microbiology
Volume43
Issue number5
DOIs
Publication statusPublished - 2005 May 1

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Acinetobacter baumannii
Imipenem
Korea
Disease Outbreaks
Cephamycins
Monobactams
Carbapenems
Pulsed Field Gel Electrophoresis
Urology
Isoelectric Focusing
Multiple Drug Resistance
Aminoglycosides
Cephalosporins
Neurology
Complex Mixtures
Penicillins
Intensive Care Units
Clone Cells
Medicine
Phenotype

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Jeon, Byung Chan ; Jeong, Seok Hoon ; Bae, Il Kwon ; Kwon, Su Bong ; Lee, Kyungwon ; Young, Dongeun ; Lee, Jung Hun ; Song, Jae Seok ; Lee, Sang Hee. / Investigation of a nosocomial outbreak of imipenem-resistant Acinetobacter baumannii producing the OXA-23 β-lactamase in Korea. In: Journal of Clinical Microbiology. 2005 ; Vol. 43, No. 5. pp. 2241-2245.
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Investigation of a nosocomial outbreak of imipenem-resistant Acinetobacter baumannii producing the OXA-23 β-lactamase in Korea. / Jeon, Byung Chan; Jeong, Seok Hoon; Bae, Il Kwon; Kwon, Su Bong; Lee, Kyungwon; Young, Dongeun; Lee, Jung Hun; Song, Jae Seok; Lee, Sang Hee.

In: Journal of Clinical Microbiology, Vol. 43, No. 5, 01.05.2005, p. 2241-2245.

Research output: Contribution to journalArticle

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