Using fluorescein isothiocyanate (FITC)-conjugated H-2Kb CTL epitope (SIINFEKL) as a model system, we investigated the antigen delivery route by pH-sensitive liposomes in vivo. Fluorescence was initially detected in lymph nodes at 3 h after immunization, and its intensity reached a peak value in superticial inguinal lymph node at 9 h. No trace could be detected in spleen even with prolonged monitoring for up to 24 h. These results strongly suggest that the presentation of CTL-peptide antigen vehicled by pH-sensitive liposomes exclusively occurs in lymph nodes. In mice immunized with the H-2Kb CTL epitope encapsulated pH-sensitive liposomes, peptide-specific CTL response was detected at day 3. The response was strongly augmented by the second immunization and persisted up to at least 45 days. These results suggest that pH-sensitive liposome formula functions as a potential adjuvant of peptide antigens and is useful for the induction of antigen specific CTLsv in vivo.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2002|
Bibliographical noteFunding Information:
This work was supported, in part, by Grant M1-00-15-00-0030-01-A21-00-027-0-0 from the Bioproducts and Biotechnology Research Center and by Grant 1999G0104 from the Korea Science and Engineering Foundation through the Bioproducts Research Center.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology