Investigation of live and dead status of airborne bacteria using UVAPS with LIVE/DEAD® BacLight Kit

Ali Mohamadi Nasrabadi, Sanggwon An, Soon Bark Kwon, Jungho Hwang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although the amount of allergens and toxins being originated from viable bacteria is higher than that from dead bacteria, monitoring concentrations of dead bacteria as well as live bacteria is an important issue related to human health. The ultraviolet aerodynamic particle sizer (UVAPS) is an instrument to measure the size distribution of only viable bioaerosols. Therefore, the main objective of this study is to detect percentages of dead as well as live airborne bacteria by using a LIVE/DEAD BacLight Bacterial Viability Kit (L7012, Molecular probes, USA) together with UVAPS. Two different bacteria species, Escherichia coli (E.coli) and Micrococcus luteus (M. luteus), were used for tests. Percentages of fluorescence emitted from these bacteria were compared with those from standard PSL and dust particles. The increased percentages for dead E. coli and dead M. luteus were 41% and 75%, respectively, while this kit did not show any effective influence on fluorescence percentages of polystyrene latex and dust particles. Field test was carried out in various indoor environments to investigate percentages of live and dead airborne bacteria.

Original languageEnglish
Pages (from-to)181-189
Number of pages9
JournalJournal of Aerosol Science
Volume115
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

aerodynamics
Bacteria
Aerodynamics
bacterium
Escherichia coli
Particles (particulate matter)
Dust
fluorescence
Fluorescence
Allergens
dust
Molecular Probes
particle
Latexes
toxin
Polystyrenes
viability
probe
Health
Monitoring

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Materials Science(all)
  • Pollution

Cite this

Nasrabadi, Ali Mohamadi ; An, Sanggwon ; Kwon, Soon Bark ; Hwang, Jungho. / Investigation of live and dead status of airborne bacteria using UVAPS with LIVE/DEAD® BacLight Kit. In: Journal of Aerosol Science. 2018 ; Vol. 115. pp. 181-189.
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Investigation of live and dead status of airborne bacteria using UVAPS with LIVE/DEAD® BacLight Kit. / Nasrabadi, Ali Mohamadi; An, Sanggwon; Kwon, Soon Bark; Hwang, Jungho.

In: Journal of Aerosol Science, Vol. 115, 01.01.2018, p. 181-189.

Research output: Contribution to journalArticle

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