Investigation of penetration force of living cell using an atomic force microscope

Eun Young Kwon, Young Tae Kim, Dae Eun Kim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Recently, the manipulation of a single cell has been receiving much attention in transgenesis, in-vitro fertilization, individual cell based diagnosis, and pharmaceutical applications. As these techniques require precise injection and manipulation of cells, issues related to penetration force arise. In this work the penetration force of living cell was studied using an atomic force microscope (AFM). L929, HeLa, 4T1, and TA3 HA II cells were used for the experiments. The results showed that the penetration force was in the range of 2~22 nN. It was also found that location of cell penetration and stiffness of the AFM cantilever affected the penetration force significantly. Furthermore, double penetration events could be detected, due to the multi-membrane layers of the cell. The findings of this work are expected to aid in the development of precision micro-medical instruments for cell manipulation and treatment.

Original languageEnglish
Pages (from-to)1932-1938
Number of pages7
JournalJournal of Mechanical Science and Technology
Volume23
Issue number7
DOIs
Publication statusPublished - 2009

Bibliographical note

Funding Information:
This research has been supported by the Intelligent Microsystem Center (IMC; http://www.microsystem. re.kr), which carries out one of the 21st century’s Frontier R&D Projects sponsored by the Korea Ministry Of Commerce, Industry and Energy.

All Science Journal Classification (ASJC) codes

  • Mechanics of Materials
  • Mechanical Engineering

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