Investigation of portable in situ fluorescence optical detection for microfluidic 3D cell culture assays

Jong Ryul Choi, Jong Hwan Sung, Michael Louis Shuler, Donghyun Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A portable fluorescence optical detection system was developed to demonstrate real-time in situ analysis of cells that are three-dimensionally cultured in an extracellular matrix under microfluidic environment. The system was designed to provide a large field of view in the lateral plane to average out cellular processes in an axial layer and simultaneously diffraction-limited axial resolution. In this proof-of-concept study, the detection system was applied to quantitative analyses of short-term measurements of cell staining and cell cytotoxicity and long-term monitoring of a cell-invasion assay. For assays, colon cancer cells were cultured in a Matrigel or alginate matrix. The measured data were largely consistent with predicted results and revealed quantitatively cell dynamics specific to 3D cell cultures. The detection system has a potential as a single package to investigate 3D cultures in a microfluidic system.

Original languageEnglish
Pages (from-to)1374-1376
Number of pages3
JournalOptics Letters
Volume35
Issue number9
DOIs
Publication statusPublished - 2010 May 1

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fluorescence
cells
staining
matrices
cultured cells
field of view
cancer
diffraction

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Choi, Jong Ryul ; Sung, Jong Hwan ; Shuler, Michael Louis ; Kim, Donghyun. / Investigation of portable in situ fluorescence optical detection for microfluidic 3D cell culture assays. In: Optics Letters. 2010 ; Vol. 35, No. 9. pp. 1374-1376.
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Investigation of portable in situ fluorescence optical detection for microfluidic 3D cell culture assays. / Choi, Jong Ryul; Sung, Jong Hwan; Shuler, Michael Louis; Kim, Donghyun.

In: Optics Letters, Vol. 35, No. 9, 01.05.2010, p. 1374-1376.

Research output: Contribution to journalArticle

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