Investigation on achieving super-resolution by solid immersion lens based STED microscopy

Wan Chin Kim, Hyungbae Moon, Won Sup Lee, Geon Lim, Guk Jong Choi, Donyoung Kang, Hyungsuk Lee, No Cheol Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The feasibility of stimulated emission depletion (STED) microscopy using a solid immersion lens was investigated. First, the theoretical feasibility of the considered system is discussed based on a vectorial field algorithm that uses a stratified medium composed of a SIL air-gap and test sample. Using the simulation, we verified that evanescent waves with much higher spatial frequencies corresponding to the high numerical aperture in the air-gap can be utilized to achieve a higher resolution than a confocal fluorescent image without a depletion beam. The simulated expectation was supported by actual imaging on two types of samples: fluorescent beads with a 20 nm diameter and an actin sample with a filamentous structure. The lateral resolution of the system was determined to be 34 nm via the imaging results on the nano-beads. The system was quite promising for achieving nano-scale surface imaging of biological samples; an even higher resolution was achieved by adjusting the wavelength and the intensity of the depletion beam.

Original languageEnglish
Pages (from-to)16629-16642
Number of pages14
JournalOptics Express
Volume25
Issue number14
DOIs
Publication statusPublished - 2017 Jul 10

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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