Investigation on biodegradable PLGA scaffold with various pore size structure for skin tissue engineering

Jun Jae Lee, Sang Gil Lee, Jongchul Park, Young Il Yang, Jeong Koo Kim

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

For tissue regeneration, highly open porous polymer matrices are required for high-density cell seeding, as well as sufficient nutrient and oxygen supply to the cells in the 3-D matrices. In this study, three types of scaffolds containing three different pore sizes (uniform-pore size, 2-layer pore size and multi-pore size) were prepared. We used human dermal fibroblast cells to investigate cell attachment and proliferation with the prepared specimens. Not only DNA quantity measurement of the cells but also the number of cells within the cross-sectional area of the scaffold was investigated. In the DNA quantity test, the multi-pore size scaffold contained a 1.77 times larger amount than the uniform-pore size scaffold for 14 days culture. For the cell-counting assessment, the multi-pore size scaffold contained about 2.24 times more cells than the uniform-pore size scaffold in the middle sectioned area for 14 days. To prevent the generation of a polymer skin layer on the surface of the scaffolds, PLGA scaffolds were fabricated by a two-step molding method. No skin layer was observed in the scaffold. Various pore size specimens tended to degrade more and faster than uniform-pore size specimens in PBS solution. Chemical treatment on the surface of the specimen enhances cytocompatibility of the scaffold.

Original languageEnglish
JournalCurrent Applied Physics
Volume7
Issue numberSUPPL.1
DOIs
Publication statusPublished - 2007 Apr 1

Fingerprint

tissue engineering
Scaffolds (biology)
Tissue engineering
Pore size
Skin
Scaffolds
porosity
cells
DNA
deoxyribonucleic acid
polylactic acid-polyglycolic acid copolymer
Oxygen supply
Tissue regeneration
fibroblasts
nutrients
polymers
Fibroblasts
inoculation
matrices
Polymer matrix

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Lee, Jun Jae ; Lee, Sang Gil ; Park, Jongchul ; Yang, Young Il ; Kim, Jeong Koo. / Investigation on biodegradable PLGA scaffold with various pore size structure for skin tissue engineering. In: Current Applied Physics. 2007 ; Vol. 7, No. SUPPL.1.
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Investigation on biodegradable PLGA scaffold with various pore size structure for skin tissue engineering. / Lee, Jun Jae; Lee, Sang Gil; Park, Jongchul; Yang, Young Il; Kim, Jeong Koo.

In: Current Applied Physics, Vol. 7, No. SUPPL.1, 01.04.2007.

Research output: Contribution to journalArticle

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