Recombinantion between RNAs associated with turnip crinkle virus is thought to occur during plus-strand synthesis at motifs resembling the 5′-ends of genomic, subgenomic and satellite RNAs. Common structural regions encompassing the motifs have been found for major crossover sites on two different minus-strand templates, with junctions preferentially located in a single-stranded region at the 3′ base of a hairpin. Base changes, deletions and compensatory alteration constructed in and around the hairpin in the region of the turnip crinkle virus genomic RNA involved in recombination support the importance of the hairpin for normal crossover site selection. This region of the genomic RNA is also important for replication of the viral genomic RNA in plants and protoplasts, suggesting a common link betwee sequences required for recombination and viral replication.
Bibliographical noteFunding Information:
We are grateful to M. Farley for technical assistance. This work was supported by grants MCB-9419303 and MCB-9315948 from the National Science Foundation to A.E.S.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology