Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells

Eun Young Shin, Do Sik Min, Ji Cheol Shin, Kyung Sun Shin, Min Soo Hyun, Kwon Soo Ha, Hun Sik Kim, Hee Yul Ahn, Eung Gook Kim

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8 Citations (Scopus)

Abstract

Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca2+. Removal of extracellular Ca2+ inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca2+ toxicity.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalFEBS Letters
Volume508
Issue number2
DOIs
Publication statusPublished - 2001 Nov 16

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Shin, E. Y., Min, D. S., Shin, J. C., Shin, K. S., Hyun, M. S., Ha, K. S., Kim, H. S., Ahn, H. Y., & Kim, E. G. (2001). Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells. FEBS Letters, 508(2), 277-281. https://doi.org/10.1016/S0014-5793(01)03059-9