Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells

Eun Young Shin, Do Sik Min, Ji Cheol Shin, Kyung Sun Shin, Min Soo Hyun, Kwon Soo Ha, Hun Sik Kim, Hee Yul Ahn, Eung Gook Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca2+. Removal of extracellular Ca2+ inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca2+ toxicity.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalFEBS Letters
Volume508
Issue number2
DOIs
Publication statusPublished - 2001 Nov 16

Fingerprint

Phospholipase D
Oxidative stress
Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Oxidative Stress
Necrosis
Cells
Hydrogen Peroxide
Vanadates
Toxicity
Chemical activation
pervanadate

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Shin, Eun Young ; Min, Do Sik ; Shin, Ji Cheol ; Shin, Kyung Sun ; Hyun, Min Soo ; Ha, Kwon Soo ; Kim, Hun Sik ; Ahn, Hee Yul ; Kim, Eung Gook. / Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells. In: FEBS Letters. 2001 ; Vol. 508, No. 2. pp. 277-281.
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abstract = "Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca2+. Removal of extracellular Ca2+ inhibited pervanadate-induced necrosis by 50{\%}. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca2+ toxicity.",
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Shin, EY, Min, DS, Shin, JC, Shin, KS, Hyun, MS, Ha, KS, Kim, HS, Ahn, HY & Kim, EG 2001, 'Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells', FEBS Letters, vol. 508, no. 2, pp. 277-281. https://doi.org/10.1016/S0014-5793(01)03059-9

Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells. / Shin, Eun Young; Min, Do Sik; Shin, Ji Cheol; Shin, Kyung Sun; Hyun, Min Soo; Ha, Kwon Soo; Kim, Hun Sik; Ahn, Hee Yul; Kim, Eung Gook.

In: FEBS Letters, Vol. 508, No. 2, 16.11.2001, p. 277-281.

Research output: Contribution to journalArticle

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AU - Shin, Eun Young

AU - Min, Do Sik

AU - Shin, Ji Cheol

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AU - Ha, Kwon Soo

AU - Kim, Hun Sik

AU - Ahn, Hee Yul

AU - Kim, Eung Gook

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AB - Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca2+. Removal of extracellular Ca2+ inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca2+ toxicity.

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