Involvement of SRSF11 in cell cycle-specific recruitment of telomerase to telomeres at nuclear speckles

Ji Hoon Lee, Sun Ah Jeong, Prabhat Khadka, Juyeong Hong, In Kwon Chung

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5 Citations (Scopus)

Abstract

Telomerase, a unique ribonucleoprotein complex that contains the telomerase reverse transcriptase (TERT), the telomerase RNA component (TERC) and the TERC-binding protein dyskerin, is required for continued cell proliferation in stem cells and cancer cells. Here we identify SRSF11 as a novel TERCbinding protein that localizes to nuclear speckles, subnuclear structures that are enriched in premessenger RNA splicing factors. SRSF11 associates with active telomerase enzyme through an interaction with TERC and directs it to nuclear speckles specifically during S phase of the cell cycle. On the other hand, a subset of telomeres is shown to be constitutively present at nuclear speckles irrespective of cell cycle phase, suggesting that nuclear speckles could be the nuclear sites for telomerase recruitment to telomeres. SRSF11 also associates with telomeres through an interaction with TRF2, which facilitates translocation of telomerase to telomeres. Depletion of SRSF11 prevents telomerase from associating with nuclear speckles and disrupts telomerase recruitment to telomeres, thereby abrogating telomere elongation by telomerase. These findings suggest that SRSF11 acts as a nuclear speckle-targeting factor that is essential for telomerase association with telomeres through the interactions with TERC and TRF2, and provides a potential target for modulating telomerase activity in cancer.

Original languageEnglish
Pages (from-to)8435-8451
Number of pages17
JournalNucleic acids research
Volume43
Issue number17
DOIs
Publication statusPublished - 2015 Sep 30

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All Science Journal Classification (ASJC) codes

  • Genetics

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