Involvement of the FoxO3a pathway in the ischemia/reperfusion injury of cardiac microvascular endothelial cells

Xu Feng Qi, Yun Jian Li, Zhuo Ying Chen, Soo Ki Kim, Kyu Jae Lee, Dong Qing Cai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

FoxO3a, a member of the forkhead transcription factors, has been demonstrated to be involved in myocardial ischemia/reperfusion (I/R) injury. Cardiac microvascular endothelial cells (CMECs) are some of the predominant cells damaged immediately after myocardial I/R injury. Despite the importance of injured CMECs in an ischemic heart, little is known about the involvement of FoxO3a in regulating CMECs injury. Thus, we used rat CMECs following simulated I/R to examine FoxO3a activation and signaling in relation to survival, the cell cycle and apoptosis in CMECs. We found that Akt negatively regulates activation of the FoxO3a pathway by phosphorylating FoxO3a in CMECs as demonstrated with an Akt inhibitor and activator. Upon I/R injury, the FoxO3a pathway was significantly activated in CMECs, which was accompanied by Akt deactivation. In parallel, the I/R of CMECs induced G1-phase arrest through p27Kip1 up-regulation and significant activation of caspase-3. Accordingly, inhibition of the FoxO3a pathway by IGF-1, an Akt activator, could significantly block the I/R-enhanced activation of p27Kip1 and caspase-3 in CMECs. Collectively, our results indicate that the FoxO3a pathway is involved in the I/R injury of CMECs at least in part through the regulation of cell cycle arrest and apoptosis, suggesting that the FoxO3a pathway may be a novel therapeutic target that protects against microvascular endothelial damage in ischemic hearts.

Original languageEnglish
Pages (from-to)242-247
Number of pages6
JournalExperimental and Molecular Pathology
Volume95
Issue number2
DOIs
Publication statusPublished - 2013 Oct

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Involvement of the FoxO3a pathway in the ischemia/reperfusion injury of cardiac microvascular endothelial cells'. Together they form a unique fingerprint.

  • Cite this