Iontophoresis and Sonophoresis Stimulate Epidermal Cytokine Expression at Energies That Do Not Provoke a Barrier Abnormality

Lamellar Body Secretion and Cytokine Expression Are Linked to Altered Epidermal Calcium Levels

Eung Ho Choi, Min Jung Kim, Byung Il Yeh, Sung Ku Ahn, Seung Hun Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We performed this study to identify whether the expression of epidermal cytokines is altered by changes in epidermal calcium content, independent of skin barrier disruption. Iontophoresis and sonophoresis with the energies that do not disrupt the skin barrier, but induce changes in the epidermal calcium gradient, were applied to the skin of hairless mice. Immediately after iontophoresis and sonophoresis, immersion in a solution containing calcium was carried out, and iontophoresis in either high- or low-calcium solutions was performed. The biopsy specimens were taken for real-time quantitative RT-PCR to detect changes in mRNA level of interleukin-1α (IL-1α), tumor necrosis factor-α (TNF-α), and transforming growth factor-β in the epidermis and for immunohistochemical stain with primary antibodies to IL-1α and TNF-α. The expression of each cytokine mRNA increased in the epidermis treated with iontophoresis and sonophoresis compared to a nontreated control as well as in tape-stripped skin used as a positive control and was lower after immersion in a high-calcium solution than in low-calcium solution. IL-1α and TNF-α immunohistochemical protein staining increased with iontophoresis at low calcium. These studies suggest that changes in epidermal calcium can directly signal expression of epidermal cytokines in vivo, independent of changes in barrier function.

Original languageEnglish
Pages (from-to)1138-1144
Number of pages7
JournalJournal of Investigative Dermatology
Volume121
Issue number5
DOIs
Publication statusPublished - 2003 Jan 1

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Iontophoresis
Cytokines
Calcium
Skin
Interleukin-1
Tumor Necrosis Factor-alpha
Immersion
Epidermis
Surgical Tape
Hairless Mouse
Messenger RNA
Biopsy
Transforming Growth Factors
Tapes
Real-Time Polymerase Chain Reaction
Coloring Agents
Staining and Labeling
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

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title = "Iontophoresis and Sonophoresis Stimulate Epidermal Cytokine Expression at Energies That Do Not Provoke a Barrier Abnormality: Lamellar Body Secretion and Cytokine Expression Are Linked to Altered Epidermal Calcium Levels",
abstract = "We performed this study to identify whether the expression of epidermal cytokines is altered by changes in epidermal calcium content, independent of skin barrier disruption. Iontophoresis and sonophoresis with the energies that do not disrupt the skin barrier, but induce changes in the epidermal calcium gradient, were applied to the skin of hairless mice. Immediately after iontophoresis and sonophoresis, immersion in a solution containing calcium was carried out, and iontophoresis in either high- or low-calcium solutions was performed. The biopsy specimens were taken for real-time quantitative RT-PCR to detect changes in mRNA level of interleukin-1α (IL-1α), tumor necrosis factor-α (TNF-α), and transforming growth factor-β in the epidermis and for immunohistochemical stain with primary antibodies to IL-1α and TNF-α. The expression of each cytokine mRNA increased in the epidermis treated with iontophoresis and sonophoresis compared to a nontreated control as well as in tape-stripped skin used as a positive control and was lower after immersion in a high-calcium solution than in low-calcium solution. IL-1α and TNF-α immunohistochemical protein staining increased with iontophoresis at low calcium. These studies suggest that changes in epidermal calcium can directly signal expression of epidermal cytokines in vivo, independent of changes in barrier function.",
author = "Choi, {Eung Ho} and Kim, {Min Jung} and Yeh, {Byung Il} and Ahn, {Sung Ku} and Lee, {Seung Hun}",
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AU - Lee, Seung Hun

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N2 - We performed this study to identify whether the expression of epidermal cytokines is altered by changes in epidermal calcium content, independent of skin barrier disruption. Iontophoresis and sonophoresis with the energies that do not disrupt the skin barrier, but induce changes in the epidermal calcium gradient, were applied to the skin of hairless mice. Immediately after iontophoresis and sonophoresis, immersion in a solution containing calcium was carried out, and iontophoresis in either high- or low-calcium solutions was performed. The biopsy specimens were taken for real-time quantitative RT-PCR to detect changes in mRNA level of interleukin-1α (IL-1α), tumor necrosis factor-α (TNF-α), and transforming growth factor-β in the epidermis and for immunohistochemical stain with primary antibodies to IL-1α and TNF-α. The expression of each cytokine mRNA increased in the epidermis treated with iontophoresis and sonophoresis compared to a nontreated control as well as in tape-stripped skin used as a positive control and was lower after immersion in a high-calcium solution than in low-calcium solution. IL-1α and TNF-α immunohistochemical protein staining increased with iontophoresis at low calcium. These studies suggest that changes in epidermal calcium can directly signal expression of epidermal cytokines in vivo, independent of changes in barrier function.

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