This study is the first report that describes Iron (16.7 mg Fe0 g-1) and Nickel (0.04 mg Ni0 g-1) nanoparticles (NPs) immobilization on the support material of an Anaerobic Fluidized Bed Reactor (AFBR-NP) and the assessment of its influence on fermentation pathway when compared with a reactor without NPs (AFBR-Control). AFBRs were operated at initial pH 6.8, 37 °C, 3.0 h Hydraulic Retention Time. Natural fermentation along with bioaugmentation with Clostridium butyricum were used as inoculation strategy. Both reactors were fed with glucose as main substrate (56 ± 6 and 21 ± 2 g COD L-1 d-1 at operational Stages I and II, respectively). 78–98% higher Volatile Fatty Acid (VFA) was observed on AFBR-NP. Metals higher availability specifically favored butyric (1.04 ± 0.37 g L-1) and acetic (1.22 ± 0.33 g L-1) acid production metabolic route on AFBR-NP. Under the same operational conditions, lactic acid was observed as main metabolite on AFBR-Control (1.23 ± 0.25 g L-1). Enzymes related to butyric (butyryl-CoA dehydrogenase, phosphate butyryltransferase, butyrate kinase) and propionic acid (pyruvate-ferredoxin oxidoreductase) metabolic routes were identified on AFBR-NP reactor biofilm. Through these results, it is possible to infer that Fe0 and Ni0 NPs could effectively improve VFA production and the fermentation process.
|Journal||Biochemical Engineering Journal|
|Publication status||Published - 2022 Nov|
Bibliographical noteFunding Information:
The present study received financial support of São Paulo Research Foundation ( FAPESP ) (Processes Numbers: 2015/06246-7 ; 2017/23008-8 and 2018/26470-7 ); Coordination for the Improvement of Higher Education Personnel ( CAPES ) (Financial code: 001 ) and Brazilian National Council for Scientific and Technological Development ( CNPq ).
© 2022 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Biomedical Engineering