Irreversible denaturation of DNA: A method to precisely control the optical and thermo-optic properties of DNA thin solid films

Hayoung Jeong, Paulson Bjorn, Seongjin Hong, Seunguk Cheon, Kyunghwan Oh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The denaturation of double-stranded deoxyribonucleic acid (ds-DNA) has been well known to break nucleobase bonds, resulting in single-stranded deoxyribonucleic acid (ss-DNA) in solutions, which can recombine to form ds-DNA in a reversible manner. We developed an efficient process to irreversibly maintain various DNA denaturation levels in thin solid films in order to investigate the impacts of the denaturation on the optical properties of DNA films. By adding NaOH in an aqueous solution of salmon testis DNA, we flexibly controlled the level of denaturation in the solution, which was then spin-coated on Si and silica substrates to irreversibly bind ss-DNAs in a thin solid film. The denaturation of DNA in thin solid films was experimentally confirmed by ultravioletvisible and Fourier transform infrared spectroscopic investigations, whose level could be controlled by the NaOH content in the aqueous solution precursor. By this irreversible denaturation process, we developed a new method to flexibly vary the refractive index of DNA thin solid films in a wide range of Δn > 0.02 in the visible to nearinfrared range. Thermo-optic coefficients dn/dT of the films were also experimentally measured in the temperature range from 40°C to 90°C to confirm the significant impacts of denaturation. Detailed thin film processes and optical characterizations are discussed.

Original languageEnglish
Pages (from-to)919-924
Number of pages6
JournalPhotonics Research
Volume6
Issue number9
DOIs
Publication statusPublished - 2018 Sep 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this