Is laser repetition rate important for two-photon light sheet microscopy?

Federico M. Gasparoli, Adrià Escobet-Montalbán, Jason Early, Graham D. Bruce, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate the thermal advantages of using low repetition rate, high peak power lasers for imaging in two-photon light sheet microscopy using a Bessel light beam. We compare the use of two ultrashort pulsed lasers in such an imaging system: a high repetition rate source operating at 80MHz and a low repetition rate source operating at 1 MHz. The low repetition rate laser requires approximately one order of magnitude lower average power than the high repetition rate source to yield the same fluorescent signal. These lasers are used to image Zebrafish larvae and record their heart rates. The data show heart rate values 30% in excess of the ground truth baseline value when imaged with the high repetition rate source due to deleterious heating, whereas the low repetition rate source yields data only a few percent above this ground truth value.

Original languageEnglish
Pages (from-to)2935-2942
Number of pages8
JournalOSA Continuum
Volume3
Issue number10
DOIs
Publication statusPublished - 2020

Bibliographical note

Publisher Copyright:
© 2020 OSA - The Optical Society. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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