Is the Affinity Column-Mediated Immunoassay Method Suitable as an Alternative to the Microparticle Enzyme Immunoassay Method as a Blood Tacrolimus Assay?

M. K. Ju; H. K. Chang; H. J. Kim; K. H. Huh; H. J. Ahn; M. S. Kim; S. I. Kim; Y. S. Kim

doi:10.1016/j.transproceed.2008.04.019

Is the Affinity Column-Mediated Immunoassay Method Suitable as an Alternative to the Microparticle Enzyme Immunoassay Method as a Blood Tacrolimus Assay?

M. K. Ju, H. K. Chang, H. J. Kim, K. H. Huh, H. J. Ahn, M. S. Kim, S. I. Kim, Y. S. Kim

Department of Surgery

Research output: Contribution to journal › Article

18 Citations (Scopus)

Abstract

Background: Tacrolimus is a potent immunosuppressive drug used in organ transplantation. Because of its substantial toxic effects, narrow therapeutic index, and interindividual pharmacokinetic variability, therapeutic drug monitoring of whole-blood tacrolimus concentrations has been recommended. We investigated the comparability of the results of 2 immunoassay systems, affinity column-mediated immunoassay (ACMIA) and microparticle enzyme immunoassay (MEIA), comparing differences in the tacrolimus concentrations measured by the 2 methods in relation to the hematologic and biochemical values of hepatic and renal functions. Methods: A total of 154 samples from kidney or liver transplant recipients were subjected to Dimension RxL HM with a tacrolimus Flex reagent cartilage for the ACMIA method and IMx tacrolimus II for the MEIA method. Results: Tacrolimus concentrations measured by the ACMIA method (n = 154) closely correlated with those measured by the MEIA method (r = 0.84). The Bland-Altman plot using concentration differences between the 2 methods and the average of the 2 methods showed no specific trends. The tacrolimus levels determined by both the MEIA method and the ACMIA method were not influenced by hematocrit levels, but the difference between the 2 methods (ACMIA - MEIA) tended to be larger in low hematocrit samples (P < .001). Conclusion: The ACMIA method used for a tacrolimus assay is precise and has advantages, including the lack of a required pretreatment procedure. Furthermore, it is only slightly influenced by the hematologic or biochemical status of the samples.

Original language	English
Pages (from-to)	3673-3678
Number of pages	6
Journal	Transplantation Proceedings
Volume	40
Issue number	10
DOIs	https://doi.org/10.1016/j.transproceed.2008.04.019
Publication status	Published - 2008 Dec 1

Fingerprint

Tacrolimus

Immunoenzyme Techniques

Immunoassay

Hematocrit

Kidney

Drug Monitoring

Poisons

Liver

Organ Transplantation

Therapeutic Uses

Immunosuppressive Agents

Cartilage

Pharmacokinetics

All Science Journal Classification (ASJC) codes

Surgery
Transplantation

Cite this

Ju, M. K., Chang, H. K., Kim, H. J., Huh, K. H., Ahn, H. J., Kim, M. S., ... Kim, Y. S. (2008). Is the Affinity Column-Mediated Immunoassay Method Suitable as an Alternative to the Microparticle Enzyme Immunoassay Method as a Blood Tacrolimus Assay? Transplantation Proceedings, 40(10), 3673-3678. https://doi.org/10.1016/j.transproceed.2008.04.019

Ju, M. K. ; Chang, H. K. ; Kim, H. J. ; Huh, K. H. ; Ahn, H. J. ; Kim, M. S. ; Kim, S. I. ; Kim, Y. S. / Is the Affinity Column-Mediated Immunoassay Method Suitable as an Alternative to the Microparticle Enzyme Immunoassay Method as a Blood Tacrolimus Assay?. In: Transplantation Proceedings. 2008 ; Vol. 40, No. 10. pp. 3673-3678.

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title = "Is the Affinity Column-Mediated Immunoassay Method Suitable as an Alternative to the Microparticle Enzyme Immunoassay Method as a Blood Tacrolimus Assay?",

abstract = "Background: Tacrolimus is a potent immunosuppressive drug used in organ transplantation. Because of its substantial toxic effects, narrow therapeutic index, and interindividual pharmacokinetic variability, therapeutic drug monitoring of whole-blood tacrolimus concentrations has been recommended. We investigated the comparability of the results of 2 immunoassay systems, affinity column-mediated immunoassay (ACMIA) and microparticle enzyme immunoassay (MEIA), comparing differences in the tacrolimus concentrations measured by the 2 methods in relation to the hematologic and biochemical values of hepatic and renal functions. Methods: A total of 154 samples from kidney or liver transplant recipients were subjected to Dimension RxL HM with a tacrolimus Flex reagent cartilage for the ACMIA method and IMx tacrolimus II for the MEIA method. Results: Tacrolimus concentrations measured by the ACMIA method (n = 154) closely correlated with those measured by the MEIA method (r = 0.84). The Bland-Altman plot using concentration differences between the 2 methods and the average of the 2 methods showed no specific trends. The tacrolimus levels determined by both the MEIA method and the ACMIA method were not influenced by hematocrit levels, but the difference between the 2 methods (ACMIA - MEIA) tended to be larger in low hematocrit samples (P < .001). Conclusion: The ACMIA method used for a tacrolimus assay is precise and has advantages, including the lack of a required pretreatment procedure. Furthermore, it is only slightly influenced by the hematologic or biochemical status of the samples.",

author = "Ju, {M. K.} and Chang, {H. K.} and Kim, {H. J.} and Huh, {K. H.} and Ahn, {H. J.} and Kim, {M. S.} and Kim, {S. I.} and Kim, {Y. S.}",

year = "2008",

month = "12",

day = "1",

doi = "10.1016/j.transproceed.2008.04.019",

language = "English",

volume = "40",

pages = "3673--3678",

journal = "Transplantation Proceedings",

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Ju, MK, Chang, HK, Kim, HJ, Huh, KH, Ahn, HJ, Kim, MS, Kim, SI & Kim, YS 2008, 'Is the Affinity Column-Mediated Immunoassay Method Suitable as an Alternative to the Microparticle Enzyme Immunoassay Method as a Blood Tacrolimus Assay?', Transplantation Proceedings, vol. 40, no. 10, pp. 3673-3678. https://doi.org/10.1016/j.transproceed.2008.04.019