IS6110-restriction fragment length polymorphism and spoligotyping analysis of Mycobacterium tuberculosis clinical isolates for investigating epidemiologic distribution in Korea

Go Eun Choi, Mi Hee Jang, Eun Ju Song, Seokhoon Jeong, Jae Seok Kim, Wee Gyo Lee, Young Uh, Kyoung Ho Roh, Hye Soo Lee, Jong Hee Shin, Nam Hee Ryoo, Young Ree Kim, Joseph Jeong, Jee Hee Kim, Sun Min Lee, Jongyoun Yi, Sang Hyun Hwang, Hyung Hoi Kim, Eun Yup Lee, Chulhun L. Chang & 2 others Moon Bum Kim, Yeong Dae Kim

Research output: Contribution to journalArticle

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Abstract

The Beijing family of Mycobacterium tuberculosis has been emerging in the world.However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates ofM. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M.tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview ofthe distribution of genotypes of M. tuberculosis in Korea. These findings indicate thatwe have to pay more attention to control of M. tuberculosis strains associated with theBeijing family.

Original languageEnglish
Pages (from-to)1716-1721
Number of pages6
JournalJournal of Korean medical science
Volume25
Issue number12
DOIs
Publication statusPublished - 2010 Dec 1

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Korea
Mycobacterium tuberculosis
Restriction Fragment Length Polymorphisms
Tuberculosis
Drug Resistance
Genotype
Phenotype
Beijing
Population

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Choi, Go Eun ; Jang, Mi Hee ; Song, Eun Ju ; Jeong, Seokhoon ; Kim, Jae Seok ; Lee, Wee Gyo ; Uh, Young ; Roh, Kyoung Ho ; Lee, Hye Soo ; Shin, Jong Hee ; Ryoo, Nam Hee ; Kim, Young Ree ; Jeong, Joseph ; Kim, Jee Hee ; Lee, Sun Min ; Yi, Jongyoun ; Hwang, Sang Hyun ; Kim, Hyung Hoi ; Lee, Eun Yup ; Chang, Chulhun L. ; Kim, Moon Bum ; Kim, Yeong Dae. / IS6110-restriction fragment length polymorphism and spoligotyping analysis of Mycobacterium tuberculosis clinical isolates for investigating epidemiologic distribution in Korea. In: Journal of Korean medical science. 2010 ; Vol. 25, No. 12. pp. 1716-1721.
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abstract = "The Beijing family of Mycobacterium tuberculosis has been emerging in the world.However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates ofM. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M.tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview ofthe distribution of genotypes of M. tuberculosis in Korea. These findings indicate thatwe have to pay more attention to control of M. tuberculosis strains associated with theBeijing family.",
author = "Choi, {Go Eun} and Jang, {Mi Hee} and Song, {Eun Ju} and Seokhoon Jeong and Kim, {Jae Seok} and Lee, {Wee Gyo} and Young Uh and Roh, {Kyoung Ho} and Lee, {Hye Soo} and Shin, {Jong Hee} and Ryoo, {Nam Hee} and Kim, {Young Ree} and Joseph Jeong and Kim, {Jee Hee} and Lee, {Sun Min} and Jongyoun Yi and Hwang, {Sang Hyun} and Kim, {Hyung Hoi} and Lee, {Eun Yup} and Chang, {Chulhun L.} and Kim, {Moon Bum} and Kim, {Yeong Dae}",
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Choi, GE, Jang, MH, Song, EJ, Jeong, S, Kim, JS, Lee, WG, Uh, Y, Roh, KH, Lee, HS, Shin, JH, Ryoo, NH, Kim, YR, Jeong, J, Kim, JH, Lee, SM, Yi, J, Hwang, SH, Kim, HH, Lee, EY, Chang, CL, Kim, MB & Kim, YD 2010, 'IS6110-restriction fragment length polymorphism and spoligotyping analysis of Mycobacterium tuberculosis clinical isolates for investigating epidemiologic distribution in Korea', Journal of Korean medical science, vol. 25, no. 12, pp. 1716-1721. https://doi.org/10.3346/jkms.2010.25.12.1716

IS6110-restriction fragment length polymorphism and spoligotyping analysis of Mycobacterium tuberculosis clinical isolates for investigating epidemiologic distribution in Korea. / Choi, Go Eun; Jang, Mi Hee; Song, Eun Ju; Jeong, Seokhoon; Kim, Jae Seok; Lee, Wee Gyo; Uh, Young; Roh, Kyoung Ho; Lee, Hye Soo; Shin, Jong Hee; Ryoo, Nam Hee; Kim, Young Ree; Jeong, Joseph; Kim, Jee Hee; Lee, Sun Min; Yi, Jongyoun; Hwang, Sang Hyun; Kim, Hyung Hoi; Lee, Eun Yup; Chang, Chulhun L.; Kim, Moon Bum; Kim, Yeong Dae.

In: Journal of Korean medical science, Vol. 25, No. 12, 01.12.2010, p. 1716-1721.

Research output: Contribution to journalArticle

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T1 - IS6110-restriction fragment length polymorphism and spoligotyping analysis of Mycobacterium tuberculosis clinical isolates for investigating epidemiologic distribution in Korea

AU - Choi, Go Eun

AU - Jang, Mi Hee

AU - Song, Eun Ju

AU - Jeong, Seokhoon

AU - Kim, Jae Seok

AU - Lee, Wee Gyo

AU - Uh, Young

AU - Roh, Kyoung Ho

AU - Lee, Hye Soo

AU - Shin, Jong Hee

AU - Ryoo, Nam Hee

AU - Kim, Young Ree

AU - Jeong, Joseph

AU - Kim, Jee Hee

AU - Lee, Sun Min

AU - Yi, Jongyoun

AU - Hwang, Sang Hyun

AU - Kim, Hyung Hoi

AU - Lee, Eun Yup

AU - Chang, Chulhun L.

AU - Kim, Moon Bum

AU - Kim, Yeong Dae

PY - 2010/12/1

Y1 - 2010/12/1

N2 - The Beijing family of Mycobacterium tuberculosis has been emerging in the world.However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates ofM. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M.tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview ofthe distribution of genotypes of M. tuberculosis in Korea. These findings indicate thatwe have to pay more attention to control of M. tuberculosis strains associated with theBeijing family.

AB - The Beijing family of Mycobacterium tuberculosis has been emerging in the world.However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates ofM. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M.tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview ofthe distribution of genotypes of M. tuberculosis in Korea. These findings indicate thatwe have to pay more attention to control of M. tuberculosis strains associated with theBeijing family.

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DO - 10.3346/jkms.2010.25.12.1716

M3 - Article

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EP - 1721

JO - Journal of Korean Medical Science

JF - Journal of Korean Medical Science

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