Isolating bronchial epithelial cell preparations from gross lung specimens

Yong Jae Kwon, Seog Joo Lee, Jae Soo Koh, Young-Joon Kim, Jong Ho Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Tissue microdissection is appropriate for separating pure cells from heterogeneous tissues. Recently, we have focused on whole genome DNA methylation patterns of lung squamous cell carcinoma (SCC) and needed to obtain the appropriate counterpart cells of lung SCC. However, in some regions of human tissues, such as in bronchial epithelium, it is difficult to apply tissue microdissection as a means to isolate pure cells from a heterogenous mixture of cells. Accordingly, we developed the pop brush method to retrieve sufficient amounts of pure bronchial epithelium from gross lung specimens, and this method enables us to study epigenetic variations of lung SCC.

Original languageEnglish
Pages (from-to)496-499
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume45
Issue number9
DOIs
Publication statusPublished - 2009 Sep 1

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Epithelial Cells
Squamous Cell Carcinoma
Lung
Microdissection
Epithelium
DNA Methylation
Epigenomics
Genome

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Kwon, Yong Jae ; Lee, Seog Joo ; Koh, Jae Soo ; Kim, Young-Joon ; Park, Jong Ho. / Isolating bronchial epithelial cell preparations from gross lung specimens. In: In Vitro Cellular and Developmental Biology - Animal. 2009 ; Vol. 45, No. 9. pp. 496-499.
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Isolating bronchial epithelial cell preparations from gross lung specimens. / Kwon, Yong Jae; Lee, Seog Joo; Koh, Jae Soo; Kim, Young-Joon; Park, Jong Ho.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 45, No. 9, 01.09.2009, p. 496-499.

Research output: Contribution to journalArticle

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