Isolation and characterization of a cDNA encoding a mammalian cathepsin L-like cysteine proteinase from Acanthamoeba healyi.

Yeon Chul Hong, Mi Yul Hwang, Ho Cheol Yun, Hak Sun Yu, Hyun Hee Kong, Tai Soon Yong, Dong Il Chung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have cloned a cDNA encoding a cysteine proteinase of the Acanthamoeba healyi OC-3A strain isolated from the brain of a granulomatous amoebic encephalitis patient. A DNA probe for an A. healyi cDNA library screening was amplified by PCR using degenerate oligonucleotide primers designed on the basis of conserved amino acids franking the active sites of cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteinases. Cysteine proteinase gene of A. healyi (AhCP1) was composed of 330 amino acids with signal sequence, a proposed pro-domain and a predicted active site made up of the catalytic residues. Cys25, His159, and Asn175. Deduced amino acid sequence analysis indicates that AhCP1 belong to ERFNIN subfamily of C1 peptidases. By Northern blot analysis, no direct correlation was observed between AhCP1 mRNA expression and virulence of Acanthamoeba, but the gene was expressed at higher level in amoebae isolated from soil than amoeba from clinical samples. These findings raise the possibility that Ahcp1 protein may play a role in protein metabolism and digestion of phagocytosed bacteria or host tissue debris rather than in invasion of amoebae into host tissue.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalThe Korean journal of parasitology
Volume40
Issue number1
DOIs
Publication statusPublished - 2002 Mar

Fingerprint

Acanthamoeba
Cathepsin L
Amoeba
Cysteine Proteases
Complementary DNA
Catalytic Domain
Amino Acids
DNA Primers
Asparagine
Protein Sequence Analysis
DNA Probes
Encephalitis
Protein Sorting Signals
Gene Library
Phagocytosis
Northern Blotting
Proteolysis
Genes
Cysteine
Virulence

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Hong, Yeon Chul ; Hwang, Mi Yul ; Yun, Ho Cheol ; Yu, Hak Sun ; Kong, Hyun Hee ; Yong, Tai Soon ; Chung, Dong Il. / Isolation and characterization of a cDNA encoding a mammalian cathepsin L-like cysteine proteinase from Acanthamoeba healyi. In: The Korean journal of parasitology. 2002 ; Vol. 40, No. 1. pp. 17-24.
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Isolation and characterization of a cDNA encoding a mammalian cathepsin L-like cysteine proteinase from Acanthamoeba healyi. / Hong, Yeon Chul; Hwang, Mi Yul; Yun, Ho Cheol; Yu, Hak Sun; Kong, Hyun Hee; Yong, Tai Soon; Chung, Dong Il.

In: The Korean journal of parasitology, Vol. 40, No. 1, 03.2002, p. 17-24.

Research output: Contribution to journalArticle

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