Isolation and characterization of a homeodomain-leucine zipper gene, Gmh1, from soybean somatic embryo

Yong Hwan Moon, Dongsu Choi, Joon Chul Kim, Tae Jin Han, Sung Ho Cho, Woo Taek Kim, Kwang Woong Lee

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Abstract

By screening a soybean somatic embryo cDNA library with a Arabidopsis thaliana homeodomain-leucine zipper (HD-Zip) cDNA clone as a probe, we isolated a cDNA clone, Glycine max homeobox gene 1 (Gmh1), encoding a polypeptide which contains a homeodomain and a closely linked leucine zipper motif. In both nucleotide and deduced amino acid sequences, Gmh1 was highly homologous to two Arabidopsis thaliana HD-Zip genes and a tomato HD-Zip gene in HD-Zip motif. Genomic Southern blot analysis indicated that Gmh1 is a single copy gene or a member of a small gene family. Also, genomic DNA of Gmh1 has three introns, 242 bp, 261 bp and 66 bp long, respectively, in the coding region. RT-PCR and Southern hybridization revealed that Gmh1 is expressed at all organs including stems, leaves, roots, flowers, seeds and somatic embryos. These results suggest that Gmh1 protein may act as a transcriptional activator during somatic embryogenesis and seed development as well as during the development of other organs in soybean.

Original languageEnglish
Pages (from-to)366-373
Number of pages8
JournalMolecules and Cells
Volume6
Issue number3
Publication statusPublished - 1996 Jun 30

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All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Moon, Y. H., Choi, D., Kim, J. C., Han, T. J., Cho, S. H., Kim, W. T., & Lee, K. W. (1996). Isolation and characterization of a homeodomain-leucine zipper gene, Gmh1, from soybean somatic embryo. Molecules and Cells, 6(3), 366-373.