Isolation and characterization of a homeodomain-leucine zipper gene, Gmh1, from soybean somatic embryo

Yong Hwan Moon, Dongsu Choi, Joon Chul Kim, Tae Jin Han, Sung Ho Cho, Woo Taek Kim, Kwang Woong Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

By screening a soybean somatic embryo cDNA library with a Arabidopsis thaliana homeodomain-leucine zipper (HD-Zip) cDNA clone as a probe, we isolated a cDNA clone, Glycine max homeobox gene 1 (Gmh1), encoding a polypeptide which contains a homeodomain and a closely linked leucine zipper motif. In both nucleotide and deduced amino acid sequences, Gmh1 was highly homologous to two Arabidopsis thaliana HD-Zip genes and a tomato HD-Zip gene in HD-Zip motif. Genomic Southern blot analysis indicated that Gmh1 is a single copy gene or a member of a small gene family. Also, genomic DNA of Gmh1 has three introns, 242 bp, 261 bp and 66 bp long, respectively, in the coding region. RT-PCR and Southern hybridization revealed that Gmh1 is expressed at all organs including stems, leaves, roots, flowers, seeds and somatic embryos. These results suggest that Gmh1 protein may act as a transcriptional activator during somatic embryogenesis and seed development as well as during the development of other organs in soybean.

Original languageEnglish
Pages (from-to)366-373
Number of pages8
JournalMolecules and Cells
Volume6
Issue number3
Publication statusPublished - 1996 Jun 30

Fingerprint

Leucine Zippers
Homeobox Genes
Soybeans
Embryonic Structures
Genes
Arabidopsis
Seeds
Complementary DNA
Clone Cells
Lycopersicon esculentum
Southern Blotting
Gene Library
Introns
Embryonic Development
Amino Acid Sequence
Nucleotides
Polymerase Chain Reaction
Peptides
DNA

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Moon, Y. H., Choi, D., Kim, J. C., Han, T. J., Cho, S. H., Kim, W. T., & Lee, K. W. (1996). Isolation and characterization of a homeodomain-leucine zipper gene, Gmh1, from soybean somatic embryo. Molecules and Cells, 6(3), 366-373.
Moon, Yong Hwan ; Choi, Dongsu ; Kim, Joon Chul ; Han, Tae Jin ; Cho, Sung Ho ; Kim, Woo Taek ; Lee, Kwang Woong. / Isolation and characterization of a homeodomain-leucine zipper gene, Gmh1, from soybean somatic embryo. In: Molecules and Cells. 1996 ; Vol. 6, No. 3. pp. 366-373.
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abstract = "By screening a soybean somatic embryo cDNA library with a Arabidopsis thaliana homeodomain-leucine zipper (HD-Zip) cDNA clone as a probe, we isolated a cDNA clone, Glycine max homeobox gene 1 (Gmh1), encoding a polypeptide which contains a homeodomain and a closely linked leucine zipper motif. In both nucleotide and deduced amino acid sequences, Gmh1 was highly homologous to two Arabidopsis thaliana HD-Zip genes and a tomato HD-Zip gene in HD-Zip motif. Genomic Southern blot analysis indicated that Gmh1 is a single copy gene or a member of a small gene family. Also, genomic DNA of Gmh1 has three introns, 242 bp, 261 bp and 66 bp long, respectively, in the coding region. RT-PCR and Southern hybridization revealed that Gmh1 is expressed at all organs including stems, leaves, roots, flowers, seeds and somatic embryos. These results suggest that Gmh1 protein may act as a transcriptional activator during somatic embryogenesis and seed development as well as during the development of other organs in soybean.",
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Moon, YH, Choi, D, Kim, JC, Han, TJ, Cho, SH, Kim, WT & Lee, KW 1996, 'Isolation and characterization of a homeodomain-leucine zipper gene, Gmh1, from soybean somatic embryo', Molecules and Cells, vol. 6, no. 3, pp. 366-373.

Isolation and characterization of a homeodomain-leucine zipper gene, Gmh1, from soybean somatic embryo. / Moon, Yong Hwan; Choi, Dongsu; Kim, Joon Chul; Han, Tae Jin; Cho, Sung Ho; Kim, Woo Taek; Lee, Kwang Woong.

In: Molecules and Cells, Vol. 6, No. 3, 30.06.1996, p. 366-373.

Research output: Contribution to journalArticle

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