Isolation and characterization of a novel agar-degrading marine bacterium, Gayadomonas joobiniege gen, nov, sp. nov., from the Southern Sea, Korea

Won Jae Chi, Jae Seon Park, Min Jung Kwak, Jihyun F. Kim, Yong Keun Chang, Soon Kwang Hong

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An agar-degrading bacterium, designated as strain G7T, was isolated from a coastal seawater sample from Gaya Island (Gayado in Korean), Republic of Korea. The isolated strain G7T is gram-negative, rod shaped, aerobic, non-motile, and non-pigmented. A similarity search based on its 16S rRNA gene sequence revealed that it shares 95.5%, 90.6%, and 90.0% similarity with the 16S rRNA gene sequences of Catenovulum agarivorans YM01T, Algicola sagamiensis, and Bowmanella pacifica W3-3AT, respectively. Phylogenetic analyses demonstrated that strain G7T formed a distinct monophyletic clade closely related to species of the family Alteromonadaceae in the Alteromonas-like Gammaproteobacteria. The G+C content of strain G7T was 41.12 mol%. The DNA-DNA hybridization value between strain G7T and the phylogenetically closest strain YM01T was 19.63%. The genomes of G7T and YM01T had an average ANIb value of 70.00%. The predominant isoprenoid quinone of this particular strain was ubiquinone-8, whereas that of C. agarivorans YM01T was menaquinone-7. The major fatty acids of strain G7T were Iso-C15:0 (41.47%), Anteiso-C15:0 (22.99%), and C16:1ω7c/iso-C15:0 2-OH (8.85%), which were quite different from those of YM01T. Comparison of the phenotypic characteristics related to carbon utilization, enzyme production, and susceptibility to antibiotics also demonstrated that strain G7T is distinct from C. agarivorans YM01T. Based on its phenotypic, chemotaxonomic, and phylogenetic distinctiveness, strain G7T was considered a novel genus and species in the Gammaproteobacteria, for which the name Gayadomonas joobiniege gen. nov. sp. nov. (ATCC BAA-2321 = DSM25250T = KCTC23721T) is proposed.

Original languageEnglish
Pages (from-to)1509-1518
Number of pages10
JournalJournal of microbiology and biotechnology
Volume23
Issue number11
DOIs
Publication statusPublished - 2013 Nov 28

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Gammaproteobacteria
Korea
rRNA Genes
Oceans and Seas
Agar
Alteromonadaceae
Alteromonas
Bacteria
Republic of Korea
DNA
Terpenes
Base Composition
Seawater
Islands
Names
Fatty Acids
Carbon
Genome
Anti-Bacterial Agents
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

@article{cb5f49aed4574207897655681702ce84,
title = "Isolation and characterization of a novel agar-degrading marine bacterium, Gayadomonas joobiniege gen, nov, sp. nov., from the Southern Sea, Korea",
abstract = "An agar-degrading bacterium, designated as strain G7T, was isolated from a coastal seawater sample from Gaya Island (Gayado in Korean), Republic of Korea. The isolated strain G7T is gram-negative, rod shaped, aerobic, non-motile, and non-pigmented. A similarity search based on its 16S rRNA gene sequence revealed that it shares 95.5{\%}, 90.6{\%}, and 90.0{\%} similarity with the 16S rRNA gene sequences of Catenovulum agarivorans YM01T, Algicola sagamiensis, and Bowmanella pacifica W3-3AT, respectively. Phylogenetic analyses demonstrated that strain G7T formed a distinct monophyletic clade closely related to species of the family Alteromonadaceae in the Alteromonas-like Gammaproteobacteria. The G+C content of strain G7T was 41.12 mol{\%}. The DNA-DNA hybridization value between strain G7T and the phylogenetically closest strain YM01T was 19.63{\%}. The genomes of G7T and YM01T had an average ANIb value of 70.00{\%}. The predominant isoprenoid quinone of this particular strain was ubiquinone-8, whereas that of C. agarivorans YM01T was menaquinone-7. The major fatty acids of strain G7T were Iso-C15:0 (41.47{\%}), Anteiso-C15:0 (22.99{\%}), and C16:1ω7c/iso-C15:0 2-OH (8.85{\%}), which were quite different from those of YM01T. Comparison of the phenotypic characteristics related to carbon utilization, enzyme production, and susceptibility to antibiotics also demonstrated that strain G7T is distinct from C. agarivorans YM01T. Based on its phenotypic, chemotaxonomic, and phylogenetic distinctiveness, strain G7T was considered a novel genus and species in the Gammaproteobacteria, for which the name Gayadomonas joobiniege gen. nov. sp. nov. (ATCC BAA-2321 = DSM25250T = KCTC23721T) is proposed.",
author = "Chi, {Won Jae} and Park, {Jae Seon} and Kwak, {Min Jung} and Kim, {Jihyun F.} and Chang, {Yong Keun} and Hong, {Soon Kwang}",
year = "2013",
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language = "English",
volume = "23",
pages = "1509--1518",
journal = "Journal of Microbiology and Biotechnology",
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Isolation and characterization of a novel agar-degrading marine bacterium, Gayadomonas joobiniege gen, nov, sp. nov., from the Southern Sea, Korea. / Chi, Won Jae; Park, Jae Seon; Kwak, Min Jung; Kim, Jihyun F.; Chang, Yong Keun; Hong, Soon Kwang.

In: Journal of microbiology and biotechnology, Vol. 23, No. 11, 28.11.2013, p. 1509-1518.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation and characterization of a novel agar-degrading marine bacterium, Gayadomonas joobiniege gen, nov, sp. nov., from the Southern Sea, Korea

AU - Chi, Won Jae

AU - Park, Jae Seon

AU - Kwak, Min Jung

AU - Kim, Jihyun F.

AU - Chang, Yong Keun

AU - Hong, Soon Kwang

PY - 2013/11/28

Y1 - 2013/11/28

N2 - An agar-degrading bacterium, designated as strain G7T, was isolated from a coastal seawater sample from Gaya Island (Gayado in Korean), Republic of Korea. The isolated strain G7T is gram-negative, rod shaped, aerobic, non-motile, and non-pigmented. A similarity search based on its 16S rRNA gene sequence revealed that it shares 95.5%, 90.6%, and 90.0% similarity with the 16S rRNA gene sequences of Catenovulum agarivorans YM01T, Algicola sagamiensis, and Bowmanella pacifica W3-3AT, respectively. Phylogenetic analyses demonstrated that strain G7T formed a distinct monophyletic clade closely related to species of the family Alteromonadaceae in the Alteromonas-like Gammaproteobacteria. The G+C content of strain G7T was 41.12 mol%. The DNA-DNA hybridization value between strain G7T and the phylogenetically closest strain YM01T was 19.63%. The genomes of G7T and YM01T had an average ANIb value of 70.00%. The predominant isoprenoid quinone of this particular strain was ubiquinone-8, whereas that of C. agarivorans YM01T was menaquinone-7. The major fatty acids of strain G7T were Iso-C15:0 (41.47%), Anteiso-C15:0 (22.99%), and C16:1ω7c/iso-C15:0 2-OH (8.85%), which were quite different from those of YM01T. Comparison of the phenotypic characteristics related to carbon utilization, enzyme production, and susceptibility to antibiotics also demonstrated that strain G7T is distinct from C. agarivorans YM01T. Based on its phenotypic, chemotaxonomic, and phylogenetic distinctiveness, strain G7T was considered a novel genus and species in the Gammaproteobacteria, for which the name Gayadomonas joobiniege gen. nov. sp. nov. (ATCC BAA-2321 = DSM25250T = KCTC23721T) is proposed.

AB - An agar-degrading bacterium, designated as strain G7T, was isolated from a coastal seawater sample from Gaya Island (Gayado in Korean), Republic of Korea. The isolated strain G7T is gram-negative, rod shaped, aerobic, non-motile, and non-pigmented. A similarity search based on its 16S rRNA gene sequence revealed that it shares 95.5%, 90.6%, and 90.0% similarity with the 16S rRNA gene sequences of Catenovulum agarivorans YM01T, Algicola sagamiensis, and Bowmanella pacifica W3-3AT, respectively. Phylogenetic analyses demonstrated that strain G7T formed a distinct monophyletic clade closely related to species of the family Alteromonadaceae in the Alteromonas-like Gammaproteobacteria. The G+C content of strain G7T was 41.12 mol%. The DNA-DNA hybridization value between strain G7T and the phylogenetically closest strain YM01T was 19.63%. The genomes of G7T and YM01T had an average ANIb value of 70.00%. The predominant isoprenoid quinone of this particular strain was ubiquinone-8, whereas that of C. agarivorans YM01T was menaquinone-7. The major fatty acids of strain G7T were Iso-C15:0 (41.47%), Anteiso-C15:0 (22.99%), and C16:1ω7c/iso-C15:0 2-OH (8.85%), which were quite different from those of YM01T. Comparison of the phenotypic characteristics related to carbon utilization, enzyme production, and susceptibility to antibiotics also demonstrated that strain G7T is distinct from C. agarivorans YM01T. Based on its phenotypic, chemotaxonomic, and phylogenetic distinctiveness, strain G7T was considered a novel genus and species in the Gammaproteobacteria, for which the name Gayadomonas joobiniege gen. nov. sp. nov. (ATCC BAA-2321 = DSM25250T = KCTC23721T) is proposed.

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