Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-xylene

Yeon Jang Jung, Dockyu Kim, Won Bae Hyun, Young Choi Ki, Jong Chan Chae, Gerben J. Zylstra, Min Kim Young, Eungbin Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Rhodococcus sp. strain YU6 was isolated from soil for the ability to grow on o-xylene as the sole carbon and energy source. Unlike most other o-xylene-degrading bacteria, YU6 is able to grow on p-xylene. Numerous growth substrate range experiments, in addition to the ring-cleavage enzyme assay data, suggest that YU6 initially metabolizes o- and p-xylene by direct aromatic ring oxidation. This leads to the formation of dimethylcatechols, which was further degraded largely through meta-cleavage pathway. The gene encoding meta-cleavage dioxygenase enzyme was PCR cloned from genomic YU6 DNA using previously known gene sequence data from the o-xylene-degrading Rhodococcus sp. strain DK17. Subsequent sequencing of the 918-bp PCR product revealed a 98% identity to the gene, encoding methylcatechol 2,3-dioxygenase from DK17. PFGE analysis followed by Southern hybridization with the catechol 2,3-dioxygenase gene demonstrated that the gene is located on an approximately 560-kb megaplasmid, designated pJYJ1.

Original languageEnglish
Pages (from-to)325-330
Number of pages6
JournalJournal of Microbiology
Volume43
Issue number4
Publication statusPublished - 2005 Aug 1

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Rhodococcus
Dioxygenases
Genes
Catechol 2,3-Dioxygenase
Polymerase Chain Reaction
Enzyme Assays
Soil
Carbon
4-xylene
2-xylene
Bacteria
DNA
Enzymes
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Jung, Y. J., Kim, D., Hyun, W. B., Ki, Y. C., Chae, J. C., Zylstra, G. J., ... Kim, E. (2005). Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-xylene. Journal of Microbiology, 43(4), 325-330.
Jung, Yeon Jang ; Kim, Dockyu ; Hyun, Won Bae ; Ki, Young Choi ; Chae, Jong Chan ; Zylstra, Gerben J. ; Young, Min Kim ; Kim, Eungbin. / Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-xylene. In: Journal of Microbiology. 2005 ; Vol. 43, No. 4. pp. 325-330.
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abstract = "Rhodococcus sp. strain YU6 was isolated from soil for the ability to grow on o-xylene as the sole carbon and energy source. Unlike most other o-xylene-degrading bacteria, YU6 is able to grow on p-xylene. Numerous growth substrate range experiments, in addition to the ring-cleavage enzyme assay data, suggest that YU6 initially metabolizes o- and p-xylene by direct aromatic ring oxidation. This leads to the formation of dimethylcatechols, which was further degraded largely through meta-cleavage pathway. The gene encoding meta-cleavage dioxygenase enzyme was PCR cloned from genomic YU6 DNA using previously known gene sequence data from the o-xylene-degrading Rhodococcus sp. strain DK17. Subsequent sequencing of the 918-bp PCR product revealed a 98{\%} identity to the gene, encoding methylcatechol 2,3-dioxygenase from DK17. PFGE analysis followed by Southern hybridization with the catechol 2,3-dioxygenase gene demonstrated that the gene is located on an approximately 560-kb megaplasmid, designated pJYJ1.",
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Jung, YJ, Kim, D, Hyun, WB, Ki, YC, Chae, JC, Zylstra, GJ, Young, MK & Kim, E 2005, 'Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-xylene', Journal of Microbiology, vol. 43, no. 4, pp. 325-330.

Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-xylene. / Jung, Yeon Jang; Kim, Dockyu; Hyun, Won Bae; Ki, Young Choi; Chae, Jong Chan; Zylstra, Gerben J.; Young, Min Kim; Kim, Eungbin.

In: Journal of Microbiology, Vol. 43, No. 4, 01.08.2005, p. 325-330.

Research output: Contribution to journalArticle

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Jung YJ, Kim D, Hyun WB, Ki YC, Chae JC, Zylstra GJ et al. Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-xylene. Journal of Microbiology. 2005 Aug 1;43(4):325-330.