Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1

Dong Woo Lee, You Seok Koh, Ki Jun Kim, Byung Chan Kim, Hak Jong Choi, Doo Sik Kim, Maggy T. Suhartono, Yu Ryang Pyun

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

A thermophilic microorganism, Bacillus thermoleovorans ID-1, isolated from hot springs in Indonesia, showed extracellular lipase activity and high growth rates on lipid substrates at elevated temperatures. On olive oil (1.5%, w/v) as the sole carbon source, the isolate ID-1 grew very rapidly at 65°C with its specific growth rate (2.50 h-1) and its lipase activity reached the maximum value of 520 U l-1 during the late exponential phase and then decreased. In addition to this, isolate ID-1 could grow on a variety of lipid substrates such as oils (olive oil, soybean oil and mineral oil), triglycerides (triolein, tributyrin) and emulsifiers (Tween 20, 40). The excreted lipase of ID-1 was purified 223-fold to homogeneity by ammonium sulfate precipitation, DEAE-Sephacel ion-exchange chromatography and Sephacryl S-200 gel filtration chromatography. As a result, the relative molecular mass of the lipase was determined to be 34 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme showed optimal activity at 70-75°C and pH 7.5 and exhibited 50% of its original activity after 1 h incubation at 60°C and 30 min at 70°C and its catalytic function was activated in the presence of Ca2+ or Zn2+. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)393-400
Number of pages8
JournalFEMS Microbiology Letters
Volume179
Issue number2
DOIs
Publication statusPublished - 1999 Oct 15

Fingerprint

Lipase
Bacillus
Hot Springs
Triolein
Lipids
Mineral Oil
Soybean Oil
Indonesia
Polysorbates
Ion Exchange Chromatography
Ammonium Sulfate
Growth
Sodium Dodecyl Sulfate
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Oils
Triglycerides
Carbon
Temperature
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Lee, Dong Woo ; Koh, You Seok ; Kim, Ki Jun ; Kim, Byung Chan ; Choi, Hak Jong ; Kim, Doo Sik ; Suhartono, Maggy T. ; Pyun, Yu Ryang. / Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1. In: FEMS Microbiology Letters. 1999 ; Vol. 179, No. 2. pp. 393-400.
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abstract = "A thermophilic microorganism, Bacillus thermoleovorans ID-1, isolated from hot springs in Indonesia, showed extracellular lipase activity and high growth rates on lipid substrates at elevated temperatures. On olive oil (1.5{\%}, w/v) as the sole carbon source, the isolate ID-1 grew very rapidly at 65°C with its specific growth rate (2.50 h-1) and its lipase activity reached the maximum value of 520 U l-1 during the late exponential phase and then decreased. In addition to this, isolate ID-1 could grow on a variety of lipid substrates such as oils (olive oil, soybean oil and mineral oil), triglycerides (triolein, tributyrin) and emulsifiers (Tween 20, 40). The excreted lipase of ID-1 was purified 223-fold to homogeneity by ammonium sulfate precipitation, DEAE-Sephacel ion-exchange chromatography and Sephacryl S-200 gel filtration chromatography. As a result, the relative molecular mass of the lipase was determined to be 34 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme showed optimal activity at 70-75°C and pH 7.5 and exhibited 50{\%} of its original activity after 1 h incubation at 60°C and 30 min at 70°C and its catalytic function was activated in the presence of Ca2+ or Zn2+. Copyright (C) 1999 Federation of European Microbiological Societies.",
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Lee, DW, Koh, YS, Kim, KJ, Kim, BC, Choi, HJ, Kim, DS, Suhartono, MT & Pyun, YR 1999, 'Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1', FEMS Microbiology Letters, vol. 179, no. 2, pp. 393-400. https://doi.org/10.1016/S0378-1097(99)00440-1

Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1. / Lee, Dong Woo; Koh, You Seok; Kim, Ki Jun; Kim, Byung Chan; Choi, Hak Jong; Kim, Doo Sik; Suhartono, Maggy T.; Pyun, Yu Ryang.

In: FEMS Microbiology Letters, Vol. 179, No. 2, 15.10.1999, p. 393-400.

Research output: Contribution to journalArticle

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T1 - Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1

AU - Lee, Dong Woo

AU - Koh, You Seok

AU - Kim, Ki Jun

AU - Kim, Byung Chan

AU - Choi, Hak Jong

AU - Kim, Doo Sik

AU - Suhartono, Maggy T.

AU - Pyun, Yu Ryang

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AB - A thermophilic microorganism, Bacillus thermoleovorans ID-1, isolated from hot springs in Indonesia, showed extracellular lipase activity and high growth rates on lipid substrates at elevated temperatures. On olive oil (1.5%, w/v) as the sole carbon source, the isolate ID-1 grew very rapidly at 65°C with its specific growth rate (2.50 h-1) and its lipase activity reached the maximum value of 520 U l-1 during the late exponential phase and then decreased. In addition to this, isolate ID-1 could grow on a variety of lipid substrates such as oils (olive oil, soybean oil and mineral oil), triglycerides (triolein, tributyrin) and emulsifiers (Tween 20, 40). The excreted lipase of ID-1 was purified 223-fold to homogeneity by ammonium sulfate precipitation, DEAE-Sephacel ion-exchange chromatography and Sephacryl S-200 gel filtration chromatography. As a result, the relative molecular mass of the lipase was determined to be 34 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme showed optimal activity at 70-75°C and pH 7.5 and exhibited 50% of its original activity after 1 h incubation at 60°C and 30 min at 70°C and its catalytic function was activated in the presence of Ca2+ or Zn2+. Copyright (C) 1999 Federation of European Microbiological Societies.

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