Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1

Dong Woo Lee, You Seok Koh, Ki Jun Kim, Byung Chan Kim, Hak Jong Choi, Doo Sik Kim, Maggy T. Suhartono, Yu Ryang Pyun

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Abstract

A thermophilic microorganism, Bacillus thermoleovorans ID-1, isolated from hot springs in Indonesia, showed extracellular lipase activity and high growth rates on lipid substrates at elevated temperatures. On olive oil (1.5%, w/v) as the sole carbon source, the isolate ID-1 grew very rapidly at 65°C with its specific growth rate (2.50 h-1) and its lipase activity reached the maximum value of 520 U l-1 during the late exponential phase and then decreased. In addition to this, isolate ID-1 could grow on a variety of lipid substrates such as oils (olive oil, soybean oil and mineral oil), triglycerides (triolein, tributyrin) and emulsifiers (Tween 20, 40). The excreted lipase of ID-1 was purified 223-fold to homogeneity by ammonium sulfate precipitation, DEAE-Sephacel ion-exchange chromatography and Sephacryl S-200 gel filtration chromatography. As a result, the relative molecular mass of the lipase was determined to be 34 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme showed optimal activity at 70-75°C and pH 7.5 and exhibited 50% of its original activity after 1 h incubation at 60°C and 30 min at 70°C and its catalytic function was activated in the presence of Ca2+ or Zn2+. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)393-400
Number of pages8
JournalFEMS Microbiology Letters
Volume179
Issue number2
DOIs
Publication statusPublished - 1999 Oct 15

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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    Lee, D. W., Koh, Y. S., Kim, K. J., Kim, B. C., Choi, H. J., Kim, D. S., Suhartono, M. T., & Pyun, Y. R. (1999). Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1. FEMS Microbiology Letters, 179(2), 393-400. https://doi.org/10.1016/S0378-1097(99)00440-1