Isolation and characterization of eight tumor necrosis factor-induced gene sequences from human fibroblasts

Tae H. Lee, Gene W. Lee, Edward B. Ziff, Jan Vilček

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

A λ cDNA library was prepared from polyadenylated RNA isolated from quiescent human diploid FS-4 fibroblasts stimulated with tumor necrosis factor for 3 h. Differential screening was used to isolate cDNA sequences that are stimulated by tumor necrosis factor. Eight distinct tumor necrosis factor-stimulated gene sequences (designated TSG-1, -6, -8, -12, -14, -21, -27, and -37) were partially sequenced and compared with known sequences from GenBank. TSG-1 was identical to the gene for interleukin-8. TSG-8 corresponded to the gene for monocyte chemotactic and activating factor. TSG-21 and -27 were identical to the genes for collagenase and stromelysin, respectively. The other four sequences showed no homologies with known genes. Patterns of induction of mRNAs corresponding to the eight cloned cDNAs by various cytokines, growth factors, and activators of second messenger pathways were analyzed in FS-4 cells.

Original languageEnglish
Pages (from-to)1982-1988
Number of pages7
JournalMolecular and cellular biology
Volume10
Issue number5
DOIs
Publication statusPublished - 1990 Jan 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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