Isolation and characterization of guamerin, a new human leukocyte elastase inhibitor from Hirudo nipponia

Hyo il Jung, Il Kim Seung Il Kim, K. S. Ha, C. O. Joe, Won Kang Ke Won Kang

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A new human leukocyte elastase inhibitor was extracted and purified from a Korean native leech Hirudo nipponia. The inhibitor, called guamerin, has a molecular weight of 6,110 and shows inhibition constant (K(i)) of 8.1 x 10- 14 M. It is stable at a wide range of pH from 1 to 11 and heat-stable up to 90 °C. The complete amino acid sequence of guamerin reveals a cysteine-rich polypeptide of 57 amino acid residues that shows no similarity to any known elastase inhibitors but has 51% sequence homology with hirustasin. Guamerin has identical spacing of 10 cysteine residues as antistasin-type serine proteinase inhibitors, but the P1 reactive site residue is Met36 instead of Arg. The neighboring sequence of the reactive site consists primarily of hydrophobic amino acid residues. Based on examinations of the target proteinases and the reactive site specificity, guamerin is a new low molecular weight protein that inhibits elastases.

Original languageEnglish
Pages (from-to)13879-13884
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number23
DOIs
Publication statusPublished - 1995 Jan 1

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Catalytic Domain
Pancreatic Elastase
Amino Acids
Cysteine
Molecular Weight
Molecular weight
Leeches
Serine Proteinase Inhibitors
Sequence Homology
Amino Acid Sequence
Peptide Hydrolases
Hot Temperature
Peptides
guamerin
human SERPINB1 protein
Proteins
hirustasin
antistasin

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Jung, Hyo il ; Seung Il Kim, Il Kim ; Ha, K. S. ; Joe, C. O. ; Ke Won Kang, Won Kang. / Isolation and characterization of guamerin, a new human leukocyte elastase inhibitor from Hirudo nipponia. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 23. pp. 13879-13884.
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Isolation and characterization of guamerin, a new human leukocyte elastase inhibitor from Hirudo nipponia. / Jung, Hyo il; Seung Il Kim, Il Kim; Ha, K. S.; Joe, C. O.; Ke Won Kang, Won Kang.

In: Journal of Biological Chemistry, Vol. 270, No. 23, 01.01.1995, p. 13879-13884.

Research output: Contribution to journalArticle

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