Isolation and characterization of guamerin, a new human leukocyte elastase inhibitor from Hirudo nipponia

Il Jung Hyo Il Jung, Il Kim Seung Il Kim, K. S. Ha, C. O. Joe, Won Kang Ke Won Kang

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

A new human leukocyte elastase inhibitor was extracted and purified from a Korean native leech Hirudo nipponia. The inhibitor, called guamerin, has a molecular weight of 6,110 and shows inhibition constant (K(i)) of 8.1 x 10- 14 M. It is stable at a wide range of pH from 1 to 11 and heat-stable up to 90 °C. The complete amino acid sequence of guamerin reveals a cysteine-rich polypeptide of 57 amino acid residues that shows no similarity to any known elastase inhibitors but has 51% sequence homology with hirustasin. Guamerin has identical spacing of 10 cysteine residues as antistasin-type serine proteinase inhibitors, but the P1 reactive site residue is Met36 instead of Arg. The neighboring sequence of the reactive site consists primarily of hydrophobic amino acid residues. Based on examinations of the target proteinases and the reactive site specificity, guamerin is a new low molecular weight protein that inhibits elastases.

Original languageEnglish
Pages (from-to)13879-13884
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number23
DOIs
Publication statusPublished - 1995 Jan 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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