Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: In vitro and in vivo evaluations

Jung Chul Park, Jeong Min Kim, Im Hee Jung, Jane C. Kim, Seong Ho Choi, Kyoo Sung Cho, Chang Sung Kim

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Objectives: Mesenchymal stem cells (MSC) could be isolated from healthy periodontal ligaments (PDL). The aims of this study were to isolate and characterize human PDL stem cells (hPDLSCs) from inflamed PDL tissue, and to evaluate their regenerative potential. Materials and Methods: Inflamed hPDLSCs (ihPDLSCs) were isolated from the inflamed PDL tissue obtained from intra-bony defects during flap surgery, and characterized by immunohistochemical staining, colony-forming unit assay, fluorescence-activated cell sorting, and mRNA expression in comparison with healthy hPDLSCs obtained from extracted teeth for orthodontic purpose. The proliferative potential and migratory potential was evaluated, and compared with healthy hPDLSCs. Regenerative potential was assessed by an in vivo ectopic transplantation model. Results: ihPDLSCs were successfully isolated and characterized as MSCs. Both ihPDLSCs and hPDLSCs were successfully differentiated under osteogenic/cementogenic and adipogenic microenvironment. The proliferative potential did not differ between healthy hPDLSCs and ihPDLSCs, while the migratory capacity was significantly increased in ihPDLSCs (p<0.05). Both groups exhibited new cementum-like tissue and related PDL fibre regeneration in an in vivo transplantation model. Conclusion: ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.

Original languageEnglish
Pages (from-to)721-731
Number of pages11
JournalJournal of Clinical Periodontology
Volume38
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1

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Periodontal Ligament
Stem Cells
Dental Cementum
Transplantation
Colony-Forming Units Assay
Orthodontics
In Vitro Techniques
Mesenchymal Stromal Cells
Regeneration
Tooth
Flow Cytometry
Staining and Labeling
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

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title = "Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: In vitro and in vivo evaluations",
abstract = "Objectives: Mesenchymal stem cells (MSC) could be isolated from healthy periodontal ligaments (PDL). The aims of this study were to isolate and characterize human PDL stem cells (hPDLSCs) from inflamed PDL tissue, and to evaluate their regenerative potential. Materials and Methods: Inflamed hPDLSCs (ihPDLSCs) were isolated from the inflamed PDL tissue obtained from intra-bony defects during flap surgery, and characterized by immunohistochemical staining, colony-forming unit assay, fluorescence-activated cell sorting, and mRNA expression in comparison with healthy hPDLSCs obtained from extracted teeth for orthodontic purpose. The proliferative potential and migratory potential was evaluated, and compared with healthy hPDLSCs. Regenerative potential was assessed by an in vivo ectopic transplantation model. Results: ihPDLSCs were successfully isolated and characterized as MSCs. Both ihPDLSCs and hPDLSCs were successfully differentiated under osteogenic/cementogenic and adipogenic microenvironment. The proliferative potential did not differ between healthy hPDLSCs and ihPDLSCs, while the migratory capacity was significantly increased in ihPDLSCs (p<0.05). Both groups exhibited new cementum-like tissue and related PDL fibre regeneration in an in vivo transplantation model. Conclusion: ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.",
author = "Park, {Jung Chul} and Kim, {Jeong Min} and Jung, {Im Hee} and Kim, {Jane C.} and Choi, {Seong Ho} and Cho, {Kyoo Sung} and Kim, {Chang Sung}",
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Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue : In vitro and in vivo evaluations. / Park, Jung Chul; Kim, Jeong Min; Jung, Im Hee; Kim, Jane C.; Choi, Seong Ho; Cho, Kyoo Sung; Kim, Chang Sung.

In: Journal of Clinical Periodontology, Vol. 38, No. 8, 01.08.2011, p. 721-731.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue

T2 - In vitro and in vivo evaluations

AU - Park, Jung Chul

AU - Kim, Jeong Min

AU - Jung, Im Hee

AU - Kim, Jane C.

AU - Choi, Seong Ho

AU - Cho, Kyoo Sung

AU - Kim, Chang Sung

PY - 2011/8/1

Y1 - 2011/8/1

N2 - Objectives: Mesenchymal stem cells (MSC) could be isolated from healthy periodontal ligaments (PDL). The aims of this study were to isolate and characterize human PDL stem cells (hPDLSCs) from inflamed PDL tissue, and to evaluate their regenerative potential. Materials and Methods: Inflamed hPDLSCs (ihPDLSCs) were isolated from the inflamed PDL tissue obtained from intra-bony defects during flap surgery, and characterized by immunohistochemical staining, colony-forming unit assay, fluorescence-activated cell sorting, and mRNA expression in comparison with healthy hPDLSCs obtained from extracted teeth for orthodontic purpose. The proliferative potential and migratory potential was evaluated, and compared with healthy hPDLSCs. Regenerative potential was assessed by an in vivo ectopic transplantation model. Results: ihPDLSCs were successfully isolated and characterized as MSCs. Both ihPDLSCs and hPDLSCs were successfully differentiated under osteogenic/cementogenic and adipogenic microenvironment. The proliferative potential did not differ between healthy hPDLSCs and ihPDLSCs, while the migratory capacity was significantly increased in ihPDLSCs (p<0.05). Both groups exhibited new cementum-like tissue and related PDL fibre regeneration in an in vivo transplantation model. Conclusion: ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.

AB - Objectives: Mesenchymal stem cells (MSC) could be isolated from healthy periodontal ligaments (PDL). The aims of this study were to isolate and characterize human PDL stem cells (hPDLSCs) from inflamed PDL tissue, and to evaluate their regenerative potential. Materials and Methods: Inflamed hPDLSCs (ihPDLSCs) were isolated from the inflamed PDL tissue obtained from intra-bony defects during flap surgery, and characterized by immunohistochemical staining, colony-forming unit assay, fluorescence-activated cell sorting, and mRNA expression in comparison with healthy hPDLSCs obtained from extracted teeth for orthodontic purpose. The proliferative potential and migratory potential was evaluated, and compared with healthy hPDLSCs. Regenerative potential was assessed by an in vivo ectopic transplantation model. Results: ihPDLSCs were successfully isolated and characterized as MSCs. Both ihPDLSCs and hPDLSCs were successfully differentiated under osteogenic/cementogenic and adipogenic microenvironment. The proliferative potential did not differ between healthy hPDLSCs and ihPDLSCs, while the migratory capacity was significantly increased in ihPDLSCs (p<0.05). Both groups exhibited new cementum-like tissue and related PDL fibre regeneration in an in vivo transplantation model. Conclusion: ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.

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