Drug resistance is one of the most significant impediments to successful chemotherapy of cancer. Multidrug-resistance (MDR) is characterized by decreased cellular sensitivity to anticancer agents due to the overexpression of P-glycoprotein. By employing adriamycin-resistance CL02 cancer cells, we undertook the screening for agents which were effective to multidrug-resistant cancer cells. As a result, a myxobacterial strain JW117 was selected for study since the solvent extract of cell mass of the strain was found to exhibit significant activity against the CL02 cancer cells. Cytotoxicity-guided chromatographic fractionation led to the isolation of phenalamides A1, A2 and A3. The producing organism was identified as Myxococcus stipitatus by taxonomic comparison with type strains of Myxococcus sp. as well as its morphological and physiological characteristics. Phenalamides A1, A2 and A3 were as active against drug-resistant cancer cells CL02 and CP70 as against the corresponding sensitive cells with IC50 values ranging from 0.23-0.57 μg/ml.
|Number of pages||5|
|Journal||Korean Journal of Applied Microbiology and Biotechnology|
|Publication status||Published - 2002|
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology