Isolation of adipose-derived stem cells by using a subfractionation culturing method

Tac Ghee Yi, Wang Kyun Kim, Joon Seok Choi, Seung Yong Song, Juhee Han, Ji Hye Kim, Won Serk Kim, Sang Gyu Park, Hyun Joo Lee, Yun Kyoung Cho, Sung Joo Hwang, Sun U. Song, Jong Hyuk Sung

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: Adipose-derived stem cells (ASCs) isolated from subcutaneous adipose tissue have been tested in clinical trials. However, ASCs isolated by enzyme digestion and centrifugation are heterogeneous and exhibit wide variation in regenerative potential and clinical outcomes. Therefore, we developed a new method for isolating clonal ASCs (cASCs) that does not use enzyme digestion or centrifugation steps. Research design and methods: In addition to cell surface markers and differentiation potential, we compared the mitogenic, paracrine and hair growth-promoting effects of ASCs isolated by the gradient centrifugation method (GCM) or by the new subfractionation culturing method (SCM). Results: We selected three cASCs isolated by SCM that showed high rates of proliferation. The cell surface markers expressed by ASCs isolated by GCM or SCM were very similar, and SCM-isolated ASCs could potentially differentiate into different cell lineages. However, cASC lines exhibited better mitogenic and paracrine effects than ASCs isolated by GCM. The expression of Diras3, Myb, Cdca7, Mki67, Rrm2, Cdk1 and Ccna2, which may play a key role in cASC proliferation, was upregulated in cASCs. In addition, cASCs exhibited enhanced hair growth-promoting effects in dermal papilla cells and animal experiments. Conclusions: SCM generates a highly homogeneous population of ASCs via a simple and effective procedure that can be used in therapeutic settings.

Original languageEnglish
Pages (from-to)1551-1560
Number of pages10
JournalExpert Opinion on Biological Therapy
Volume14
Issue number11
DOIs
Publication statusPublished - 2014 Nov 1

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Stem cells
Stem Cells
Centrifugation
Digestion
Differentiation Antigens
Enzymes
Subcutaneous Fat
Animals
Cell Lineage
Growth
Tissue
Research Design
Clinical Trials
Skin

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Yi, Tac Ghee ; Kim, Wang Kyun ; Choi, Joon Seok ; Song, Seung Yong ; Han, Juhee ; Kim, Ji Hye ; Kim, Won Serk ; Park, Sang Gyu ; Lee, Hyun Joo ; Cho, Yun Kyoung ; Hwang, Sung Joo ; Song, Sun U. ; Sung, Jong Hyuk. / Isolation of adipose-derived stem cells by using a subfractionation culturing method. In: Expert Opinion on Biological Therapy. 2014 ; Vol. 14, No. 11. pp. 1551-1560.
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abstract = "Objective: Adipose-derived stem cells (ASCs) isolated from subcutaneous adipose tissue have been tested in clinical trials. However, ASCs isolated by enzyme digestion and centrifugation are heterogeneous and exhibit wide variation in regenerative potential and clinical outcomes. Therefore, we developed a new method for isolating clonal ASCs (cASCs) that does not use enzyme digestion or centrifugation steps. Research design and methods: In addition to cell surface markers and differentiation potential, we compared the mitogenic, paracrine and hair growth-promoting effects of ASCs isolated by the gradient centrifugation method (GCM) or by the new subfractionation culturing method (SCM). Results: We selected three cASCs isolated by SCM that showed high rates of proliferation. The cell surface markers expressed by ASCs isolated by GCM or SCM were very similar, and SCM-isolated ASCs could potentially differentiate into different cell lineages. However, cASC lines exhibited better mitogenic and paracrine effects than ASCs isolated by GCM. The expression of Diras3, Myb, Cdca7, Mki67, Rrm2, Cdk1 and Ccna2, which may play a key role in cASC proliferation, was upregulated in cASCs. In addition, cASCs exhibited enhanced hair growth-promoting effects in dermal papilla cells and animal experiments. Conclusions: SCM generates a highly homogeneous population of ASCs via a simple and effective procedure that can be used in therapeutic settings.",
author = "Yi, {Tac Ghee} and Kim, {Wang Kyun} and Choi, {Joon Seok} and Song, {Seung Yong} and Juhee Han and Kim, {Ji Hye} and Kim, {Won Serk} and Park, {Sang Gyu} and Lee, {Hyun Joo} and Cho, {Yun Kyoung} and Hwang, {Sung Joo} and Song, {Sun U.} and Sung, {Jong Hyuk}",
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Yi, TG, Kim, WK, Choi, JS, Song, SY, Han, J, Kim, JH, Kim, WS, Park, SG, Lee, HJ, Cho, YK, Hwang, SJ, Song, SU & Sung, JH 2014, 'Isolation of adipose-derived stem cells by using a subfractionation culturing method', Expert Opinion on Biological Therapy, vol. 14, no. 11, pp. 1551-1560. https://doi.org/10.1517/14712598.2014.943661

Isolation of adipose-derived stem cells by using a subfractionation culturing method. / Yi, Tac Ghee; Kim, Wang Kyun; Choi, Joon Seok; Song, Seung Yong; Han, Juhee; Kim, Ji Hye; Kim, Won Serk; Park, Sang Gyu; Lee, Hyun Joo; Cho, Yun Kyoung; Hwang, Sung Joo; Song, Sun U.; Sung, Jong Hyuk.

In: Expert Opinion on Biological Therapy, Vol. 14, No. 11, 01.11.2014, p. 1551-1560.

Research output: Contribution to journalArticle

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T1 - Isolation of adipose-derived stem cells by using a subfractionation culturing method

AU - Yi, Tac Ghee

AU - Kim, Wang Kyun

AU - Choi, Joon Seok

AU - Song, Seung Yong

AU - Han, Juhee

AU - Kim, Ji Hye

AU - Kim, Won Serk

AU - Park, Sang Gyu

AU - Lee, Hyun Joo

AU - Cho, Yun Kyoung

AU - Hwang, Sung Joo

AU - Song, Sun U.

AU - Sung, Jong Hyuk

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Objective: Adipose-derived stem cells (ASCs) isolated from subcutaneous adipose tissue have been tested in clinical trials. However, ASCs isolated by enzyme digestion and centrifugation are heterogeneous and exhibit wide variation in regenerative potential and clinical outcomes. Therefore, we developed a new method for isolating clonal ASCs (cASCs) that does not use enzyme digestion or centrifugation steps. Research design and methods: In addition to cell surface markers and differentiation potential, we compared the mitogenic, paracrine and hair growth-promoting effects of ASCs isolated by the gradient centrifugation method (GCM) or by the new subfractionation culturing method (SCM). Results: We selected three cASCs isolated by SCM that showed high rates of proliferation. The cell surface markers expressed by ASCs isolated by GCM or SCM were very similar, and SCM-isolated ASCs could potentially differentiate into different cell lineages. However, cASC lines exhibited better mitogenic and paracrine effects than ASCs isolated by GCM. The expression of Diras3, Myb, Cdca7, Mki67, Rrm2, Cdk1 and Ccna2, which may play a key role in cASC proliferation, was upregulated in cASCs. In addition, cASCs exhibited enhanced hair growth-promoting effects in dermal papilla cells and animal experiments. Conclusions: SCM generates a highly homogeneous population of ASCs via a simple and effective procedure that can be used in therapeutic settings.

AB - Objective: Adipose-derived stem cells (ASCs) isolated from subcutaneous adipose tissue have been tested in clinical trials. However, ASCs isolated by enzyme digestion and centrifugation are heterogeneous and exhibit wide variation in regenerative potential and clinical outcomes. Therefore, we developed a new method for isolating clonal ASCs (cASCs) that does not use enzyme digestion or centrifugation steps. Research design and methods: In addition to cell surface markers and differentiation potential, we compared the mitogenic, paracrine and hair growth-promoting effects of ASCs isolated by the gradient centrifugation method (GCM) or by the new subfractionation culturing method (SCM). Results: We selected three cASCs isolated by SCM that showed high rates of proliferation. The cell surface markers expressed by ASCs isolated by GCM or SCM were very similar, and SCM-isolated ASCs could potentially differentiate into different cell lineages. However, cASC lines exhibited better mitogenic and paracrine effects than ASCs isolated by GCM. The expression of Diras3, Myb, Cdca7, Mki67, Rrm2, Cdk1 and Ccna2, which may play a key role in cASC proliferation, was upregulated in cASCs. In addition, cASCs exhibited enhanced hair growth-promoting effects in dermal papilla cells and animal experiments. Conclusions: SCM generates a highly homogeneous population of ASCs via a simple and effective procedure that can be used in therapeutic settings.

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