Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens

Byung Ho Kong, Na Ri Park, Jin Kyoung Shim, Bo Kyung Kim, Hye Jin Shin, Ji Hyun Lee, Yong Min Huh, Su Jae Lee, Se Hoon Kim, Eui Hyun Kim, Eun Kyung Park, Jong Hee Chang, Dong Seok Kim, Sun Ho Kim, Yong Kil Hong, Seok Gu Kang, Frederick F. Lang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Purpose: The existence of cancer stem cells (CSCs) in glioblastoma has been proposed. However, the unknown knowledge that is yet to be revealed is the presence of glioma CSCs (gCSCs) in correlation to each WHO grades of glioma. We approached this study with a hypothesis that specimens from high-grade gliomas would have higher isolation rate of gCSCs in comparison to those of lower-grade gliomas. Methods: The glioma specimens were obtained from patients and underwent gliomasphere assay. The gliomaspheres were chosen to be analyzed with immunocytochemisty for surface markers. Then the selected gliomaspheres were exposed to neural differentiation conditions. Lastly, we made mouse orthotopic glioma models to examine the capacity of gliomagenesis. Results: The gliomaspheres were formed in WHO grade IV (13 of 21) and III (two of nine) gliomas. Among them, WHO grade IV (11 of 13) and III (two of two) gliomaspheres showed similar surface markers to gCSCs and were capable of neural differentiation. Lastly, among the chosen cells, 10 of 11 WHO grade IV and two of two WHO grade III gliomaspheres were capable of gliomagenesis. Thus, overall, the rates of existence of gCSCs were more prominent in high-grade gliomas: 47.6 % (10 of 21) in WHO grade IV gliomas and 22.2 % (two of nine) in WHO grade III gliomas, whereas WHO grade II and I gliomas showed virtually no gCSCs. Conclusions: This trend of stage-by-stage increase of gCSCs in gliomas showed statistical significance by chi-square test linear-by-linear association. We prove that the rates of existence of gCSCs increase proportionally as the WHO grades of gliomas rise.

Original languageEnglish
Pages (from-to)217-229
Number of pages13
JournalChild's Nervous System
Volume29
Issue number2
DOIs
Publication statusPublished - 2013 Jan 1

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Neoplastic Stem Cells
Glioma

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Clinical Neurology

Cite this

Kong, B. H., Park, N. R., Shim, J. K., Kim, B. K., Shin, H. J., Lee, J. H., ... Lang, F. F. (2013). Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. Child's Nervous System, 29(2), 217-229. https://doi.org/10.1007/s00381-012-1964-9
Kong, Byung Ho ; Park, Na Ri ; Shim, Jin Kyoung ; Kim, Bo Kyung ; Shin, Hye Jin ; Lee, Ji Hyun ; Huh, Yong Min ; Lee, Su Jae ; Kim, Se Hoon ; Kim, Eui Hyun ; Park, Eun Kyung ; Chang, Jong Hee ; Kim, Dong Seok ; Kim, Sun Ho ; Hong, Yong Kil ; Kang, Seok Gu ; Lang, Frederick F. / Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. In: Child's Nervous System. 2013 ; Vol. 29, No. 2. pp. 217-229.
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title = "Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens",
abstract = "Purpose: The existence of cancer stem cells (CSCs) in glioblastoma has been proposed. However, the unknown knowledge that is yet to be revealed is the presence of glioma CSCs (gCSCs) in correlation to each WHO grades of glioma. We approached this study with a hypothesis that specimens from high-grade gliomas would have higher isolation rate of gCSCs in comparison to those of lower-grade gliomas. Methods: The glioma specimens were obtained from patients and underwent gliomasphere assay. The gliomaspheres were chosen to be analyzed with immunocytochemisty for surface markers. Then the selected gliomaspheres were exposed to neural differentiation conditions. Lastly, we made mouse orthotopic glioma models to examine the capacity of gliomagenesis. Results: The gliomaspheres were formed in WHO grade IV (13 of 21) and III (two of nine) gliomas. Among them, WHO grade IV (11 of 13) and III (two of two) gliomaspheres showed similar surface markers to gCSCs and were capable of neural differentiation. Lastly, among the chosen cells, 10 of 11 WHO grade IV and two of two WHO grade III gliomaspheres were capable of gliomagenesis. Thus, overall, the rates of existence of gCSCs were more prominent in high-grade gliomas: 47.6 {\%} (10 of 21) in WHO grade IV gliomas and 22.2 {\%} (two of nine) in WHO grade III gliomas, whereas WHO grade II and I gliomas showed virtually no gCSCs. Conclusions: This trend of stage-by-stage increase of gCSCs in gliomas showed statistical significance by chi-square test linear-by-linear association. We prove that the rates of existence of gCSCs increase proportionally as the WHO grades of gliomas rise.",
author = "Kong, {Byung Ho} and Park, {Na Ri} and Shim, {Jin Kyoung} and Kim, {Bo Kyung} and Shin, {Hye Jin} and Lee, {Ji Hyun} and Huh, {Yong Min} and Lee, {Su Jae} and Kim, {Se Hoon} and Kim, {Eui Hyun} and Park, {Eun Kyung} and Chang, {Jong Hee} and Kim, {Dong Seok} and Kim, {Sun Ho} and Hong, {Yong Kil} and Kang, {Seok Gu} and Lang, {Frederick F.}",
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Kong, BH, Park, NR, Shim, JK, Kim, BK, Shin, HJ, Lee, JH, Huh, YM, Lee, SJ, Kim, SH, Kim, EH, Park, EK, Chang, JH, Kim, DS, Kim, SH, Hong, YK, Kang, SG & Lang, FF 2013, 'Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens', Child's Nervous System, vol. 29, no. 2, pp. 217-229. https://doi.org/10.1007/s00381-012-1964-9

Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. / Kong, Byung Ho; Park, Na Ri; Shim, Jin Kyoung; Kim, Bo Kyung; Shin, Hye Jin; Lee, Ji Hyun; Huh, Yong Min; Lee, Su Jae; Kim, Se Hoon; Kim, Eui Hyun; Park, Eun Kyung; Chang, Jong Hee; Kim, Dong Seok; Kim, Sun Ho; Hong, Yong Kil; Kang, Seok Gu; Lang, Frederick F.

In: Child's Nervous System, Vol. 29, No. 2, 01.01.2013, p. 217-229.

Research output: Contribution to journalArticle

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T1 - Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens

AU - Kong, Byung Ho

AU - Park, Na Ri

AU - Shim, Jin Kyoung

AU - Kim, Bo Kyung

AU - Shin, Hye Jin

AU - Lee, Ji Hyun

AU - Huh, Yong Min

AU - Lee, Su Jae

AU - Kim, Se Hoon

AU - Kim, Eui Hyun

AU - Park, Eun Kyung

AU - Chang, Jong Hee

AU - Kim, Dong Seok

AU - Kim, Sun Ho

AU - Hong, Yong Kil

AU - Kang, Seok Gu

AU - Lang, Frederick F.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Purpose: The existence of cancer stem cells (CSCs) in glioblastoma has been proposed. However, the unknown knowledge that is yet to be revealed is the presence of glioma CSCs (gCSCs) in correlation to each WHO grades of glioma. We approached this study with a hypothesis that specimens from high-grade gliomas would have higher isolation rate of gCSCs in comparison to those of lower-grade gliomas. Methods: The glioma specimens were obtained from patients and underwent gliomasphere assay. The gliomaspheres were chosen to be analyzed with immunocytochemisty for surface markers. Then the selected gliomaspheres were exposed to neural differentiation conditions. Lastly, we made mouse orthotopic glioma models to examine the capacity of gliomagenesis. Results: The gliomaspheres were formed in WHO grade IV (13 of 21) and III (two of nine) gliomas. Among them, WHO grade IV (11 of 13) and III (two of two) gliomaspheres showed similar surface markers to gCSCs and were capable of neural differentiation. Lastly, among the chosen cells, 10 of 11 WHO grade IV and two of two WHO grade III gliomaspheres were capable of gliomagenesis. Thus, overall, the rates of existence of gCSCs were more prominent in high-grade gliomas: 47.6 % (10 of 21) in WHO grade IV gliomas and 22.2 % (two of nine) in WHO grade III gliomas, whereas WHO grade II and I gliomas showed virtually no gCSCs. Conclusions: This trend of stage-by-stage increase of gCSCs in gliomas showed statistical significance by chi-square test linear-by-linear association. We prove that the rates of existence of gCSCs increase proportionally as the WHO grades of gliomas rise.

AB - Purpose: The existence of cancer stem cells (CSCs) in glioblastoma has been proposed. However, the unknown knowledge that is yet to be revealed is the presence of glioma CSCs (gCSCs) in correlation to each WHO grades of glioma. We approached this study with a hypothesis that specimens from high-grade gliomas would have higher isolation rate of gCSCs in comparison to those of lower-grade gliomas. Methods: The glioma specimens were obtained from patients and underwent gliomasphere assay. The gliomaspheres were chosen to be analyzed with immunocytochemisty for surface markers. Then the selected gliomaspheres were exposed to neural differentiation conditions. Lastly, we made mouse orthotopic glioma models to examine the capacity of gliomagenesis. Results: The gliomaspheres were formed in WHO grade IV (13 of 21) and III (two of nine) gliomas. Among them, WHO grade IV (11 of 13) and III (two of two) gliomaspheres showed similar surface markers to gCSCs and were capable of neural differentiation. Lastly, among the chosen cells, 10 of 11 WHO grade IV and two of two WHO grade III gliomaspheres were capable of gliomagenesis. Thus, overall, the rates of existence of gCSCs were more prominent in high-grade gliomas: 47.6 % (10 of 21) in WHO grade IV gliomas and 22.2 % (two of nine) in WHO grade III gliomas, whereas WHO grade II and I gliomas showed virtually no gCSCs. Conclusions: This trend of stage-by-stage increase of gCSCs in gliomas showed statistical significance by chi-square test linear-by-linear association. We prove that the rates of existence of gCSCs increase proportionally as the WHO grades of gliomas rise.

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