Isolation of RNA aptamers targeting HER-2-overexpressing breast cancer cells using cell-SELEX

Hye Suk Kang, Yong Min Huh, Soyoun Kim, Dong ki Lee

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDA-MB-231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.

Original languageEnglish
Pages (from-to)1827-1831
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume30
Issue number8
DOIs
Publication statusPublished - 2009 Aug 1

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Nucleotide Aptamers
Cells
Biomarkers
Epidermal Growth Factor Receptor
Ligands
Molecules
Proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

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abstract = "Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDA-MB-231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.",
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Isolation of RNA aptamers targeting HER-2-overexpressing breast cancer cells using cell-SELEX. / Kang, Hye Suk; Huh, Yong Min; Kim, Soyoun; Lee, Dong ki.

In: Bulletin of the Korean Chemical Society, Vol. 30, No. 8, 01.08.2009, p. 1827-1831.

Research output: Contribution to journalArticle

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