Abstract
Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDA-MB-231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.
| Original language | English |
|---|---|
| Pages (from-to) | 1827-1831 |
| Number of pages | 5 |
| Journal | Bulletin of the Korean Chemical Society |
| Volume | 30 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2009 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Isolation of RNA aptamers targeting HER-2-overexpressing breast cancer cells using cell-SELEX. / Kang, Hye Suk; Huh, Yong Min; Kim, Soyoun; Lee, Dong ki.
In: Bulletin of the Korean Chemical Society, Vol. 30, No. 8, 01.08.2009, p. 1827-1831.Research output: Contribution to journal › Article
TY - JOUR
T1 - Isolation of RNA aptamers targeting HER-2-overexpressing breast cancer cells using cell-SELEX
AU - Kang, Hye Suk
AU - Huh, Yong Min
AU - Kim, Soyoun
AU - Lee, Dong ki
PY - 2009/8/1
Y1 - 2009/8/1
N2 - Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDA-MB-231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.
AB - Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDA-MB-231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.
UR - http://www.scopus.com/inward/record.url?scp=75649084157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=75649084157&partnerID=8YFLogxK
U2 - 10.5012/bkcs.2009.30.8.1827
DO - 10.5012/bkcs.2009.30.8.1827
M3 - Article
AN - SCOPUS:75649084157
VL - 30
SP - 1827
EP - 1831
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
SN - 0253-2964
IS - 8
ER -