Abstract
As an attempt to search for bioactive natural products exerting antiinflammatory activity, we have isolated two saponins were isolated from the aerial portion of Pleurospermum kamtschaticum (Umbelliferae) by nitrite assay activity-directed chromatographic fractionation. They were identified as saikogenin F 3-O-{β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl- (1→3)]-β-D-fucopyranoside} (buddlejasaponin IV, 1) and 3β,16β,23,28-tetrahydroxy-11α-methoxyolean-12-ene 3-O-{β-D-glucopyranosyl(1→2)-[β-D-glucopyranosyl(1→3)] -β-D-fucopyranoside} (buddlejasaponin IVa, 2). Compound 1 significantly inhibited nitric oxide (NO) production, and it also significantly decreased prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) release in the lipopolysaccharide (LPS)-activated macrophage Raw 264.7 cells whereas compound 2 was much less active. Saikogenin A (3) and -H (4) were obtained by hydrolyzing 1 and 2. Although these sapogenin showed strong NO inhibition, these effects were caused by the cytotoxic effect on Raw 264.7 cells. These results supported the notion that buddlejasaponin IV is a major inhibitors of NO, PGE2 and TNF-α production in P. kamtschaticum.
Original language | English |
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Pages (from-to) | 1668-1671 |
Number of pages | 4 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 28 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2005 Sep 1 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
- Pharmaceutical Science
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Isolation of saponins with the inhibitory effect on nitric oxide, prostaglandin E2 and tumor necrosis factor-α production from pleurospermum kamtschaticum. / Jung, Hyun Ju; Kim, Sei Gun; Nam, Jung Hwan; Park, Kwang Kyun; Chung, Won Yun; Kim, Won Bae; Lee, Kyung Tae; Won, Jong Heon; Choi, Jong Won; Park, Hee Juhn.
In: Biological and Pharmaceutical Bulletin, Vol. 28, No. 9, 01.09.2005, p. 1668-1671.Research output: Contribution to journal › Article
TY - JOUR
T1 - Isolation of saponins with the inhibitory effect on nitric oxide, prostaglandin E2 and tumor necrosis factor-α production from pleurospermum kamtschaticum
AU - Jung, Hyun Ju
AU - Kim, Sei Gun
AU - Nam, Jung Hwan
AU - Park, Kwang Kyun
AU - Chung, Won Yun
AU - Kim, Won Bae
AU - Lee, Kyung Tae
AU - Won, Jong Heon
AU - Choi, Jong Won
AU - Park, Hee Juhn
PY - 2005/9/1
Y1 - 2005/9/1
N2 - As an attempt to search for bioactive natural products exerting antiinflammatory activity, we have isolated two saponins were isolated from the aerial portion of Pleurospermum kamtschaticum (Umbelliferae) by nitrite assay activity-directed chromatographic fractionation. They were identified as saikogenin F 3-O-{β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl- (1→3)]-β-D-fucopyranoside} (buddlejasaponin IV, 1) and 3β,16β,23,28-tetrahydroxy-11α-methoxyolean-12-ene 3-O-{β-D-glucopyranosyl(1→2)-[β-D-glucopyranosyl(1→3)] -β-D-fucopyranoside} (buddlejasaponin IVa, 2). Compound 1 significantly inhibited nitric oxide (NO) production, and it also significantly decreased prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) release in the lipopolysaccharide (LPS)-activated macrophage Raw 264.7 cells whereas compound 2 was much less active. Saikogenin A (3) and -H (4) were obtained by hydrolyzing 1 and 2. Although these sapogenin showed strong NO inhibition, these effects were caused by the cytotoxic effect on Raw 264.7 cells. These results supported the notion that buddlejasaponin IV is a major inhibitors of NO, PGE2 and TNF-α production in P. kamtschaticum.
AB - As an attempt to search for bioactive natural products exerting antiinflammatory activity, we have isolated two saponins were isolated from the aerial portion of Pleurospermum kamtschaticum (Umbelliferae) by nitrite assay activity-directed chromatographic fractionation. They were identified as saikogenin F 3-O-{β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl- (1→3)]-β-D-fucopyranoside} (buddlejasaponin IV, 1) and 3β,16β,23,28-tetrahydroxy-11α-methoxyolean-12-ene 3-O-{β-D-glucopyranosyl(1→2)-[β-D-glucopyranosyl(1→3)] -β-D-fucopyranoside} (buddlejasaponin IVa, 2). Compound 1 significantly inhibited nitric oxide (NO) production, and it also significantly decreased prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) release in the lipopolysaccharide (LPS)-activated macrophage Raw 264.7 cells whereas compound 2 was much less active. Saikogenin A (3) and -H (4) were obtained by hydrolyzing 1 and 2. Although these sapogenin showed strong NO inhibition, these effects were caused by the cytotoxic effect on Raw 264.7 cells. These results supported the notion that buddlejasaponin IV is a major inhibitors of NO, PGE2 and TNF-α production in P. kamtschaticum.
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UR - http://www.scopus.com/inward/citedby.url?scp=24944459772&partnerID=8YFLogxK
U2 - 10.1248/bpb.28.1668
DO - 10.1248/bpb.28.1668
M3 - Article
C2 - 16141537
AN - SCOPUS:24944459772
VL - 28
SP - 1668
EP - 1671
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 9
ER -